Spectrometry binding toxicity

Grigoryan, H., Schopfer, L.M., Thompson, C.M., Terry, A.V., Masson, P., Lockridge, O. (2008). Mass spectrometry identifies covalent binding of soman, sarin, chlorpyrifos oxon, diisopropyl fluorophosphate, and FP-biotin to tyrosines on tubulin a potential mechanism of long term toxicity by organophos-phoms agents. Chem. Biol. Interact. April 22. (Epub ahead of print)... 

The advantages of pulsed ultrafiltration-mass spectrometry include the variety of different applications that may be carried out, the convenience of on-line screening, solution-phase screening, the ability to screen either combinatorial libraries or natural product extracts, the diversity of receptors that may be screened, and the freedom to use either volatile or non-volatile binding buffers. For metabolic and toxicity screening, flow injection analyses have the additional advantages that product feedback inhibition is prevented so that the metabolic profile more closely approximates the in vivo system ( 70). Finally, the... 

Dahl JE, Becher R, Aarstad K et al (1990) Species differences in short temn toxicity from inhalation exposure to bromobenzene. Arch Toxicol 64 370-376 Dai D, Tang J, Rose R et al (2001) Identification of variants of CYP3A4 and characterization of their abilities to metabolize testosterone and chlorpyrifos. J Pharmacol Exp Ther 299 825-831 Damsten MC, Commandeur IN, Fidder A et al (2007) Liquid chromalography/tandem mass spectrometry detection of covalent binding of acetaminophen to human scmnn albumin. Drug Metab Dispos 35 1408-1417... 

The search for biologically active compounds (natural and synthetic) continued to be a significant inspiration for organic synthesis. Asymmetric catalysis, new synthetic reactions, and advances in separation techniques (HPLC) and analytical techniques (NMR and mass spectrometry) support these advances. In 1995, Kyriacos Costa (K.C.) Nicolaou (1946- ) at Scripps Research Institute reported the total synthesis of brevetoxin B (see the figure on page 375). This toxic substance is produced by algae in red tide and is very deadly to fish. It binds to sodium channels in membranes of muscle and nerve cells producing an excessive influx of Na. ... 

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