Special Features of the Gradient

In high-performance liquid chromatography (HPLC), interactions of different strengths between the analytes on the one hand and eluent components and the stationary phase on the other hand usually occur during separation. In the case of isocratic separations, there is a predetermined, constant eluent composition, 

The HPLC Expert Possibilities mid Limitations of Modem Hi Petformance Liquid Chromatognqif,  

Positive from the user perspective is that simple gradient separations using 20-50 mm columns on conventional equipment generally prove to be no 

This fact, known as peak suppression, has the effect that in gradient separations tailing is rarely observed. Peak symmetry is about 10% better compared with an isocratic run with equivalent eluent composition (Hans-Joachim Kuss, personal communication). 

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