Spatially addressable combinatorial arrays

Several considerations drive the design and synthesis of spatially addressable, combinatorial arrays (25). The selection of building blocks and backbone... 

Parallel synthesis is the term loosely applied to the preparation of discrete compounds in the format of a spatially addressable array. It is often used in contrast to "mixture-based" combinatorial synthesis. While it is hue that the term "parallel synthesis" may encompass the preparation of a fully combinatorial multidimensional array of compounds, in practice, it most... 

In-situ photofabrication of OND arrays involves light-directed, spatially addressable, parallel (combinatorial) chemical synthesis [35,43,66-68]. A surface, usually a sihcon wafer or a glass slide, is coated with hnker molecules... 

The use of planar supports for presenting large arrays of spatially addressed molecules is one of the most powerful and versatile methods for creating combinatorial libraries.19,28-29... 

Scharn, D., Wenschuh, H., Reineke, U., Mergener, J.S., and Germeroth L. 2002. Spatially addressed synthesis of amino- and amino-oxy-substituted 1,3,5-triazine arrays on polymeric membranes. Journal of Combinatorial Chemistry, 2 361-69. 

Combinatorial libraries are prepared by the (1) parallel synthesis of arrays, (2) split-pool method, (3) biological method, or (4) spatially addressable parallel synthesis [74,78-80]. Parallel synthesis is carried out by the simultaneous synthesis of an array of different compounds. Several methods are available. In the multipin method, the peptide synthesis is carried out on polyethylene rods that have attached protected amino acids [81]. The amino acid sequence of a synthesized peptide on a particular pin depends on the order in which the amino acids are added. The number of products synthesized is the same as the number of pins. Another version of parallel synthesis, known as the teabag method, uses resin-filled bags in place of pins [74]. By pooling the resin portions from the appropriate bags, followed by redistribution and further coupling with a specific amino acid, a peptide library can be synthesized. The SPOT method uses a cellulose paper membrane as a solid support, which acts as an open reactor. Respective reagent solutions are pipetted onto several spots to synthesize as many peptides as the spots chosen [74,82]. 

Alternatives. A sophisticated robot for 2D and 3D spatially addressed arrays for the high-throughput automated synthesis of combinatorial libraries was designed by Selectide scientists. A directed sort and combine synthesis with spatially arranged arrays used macroscopic supports (Mimotopes Lanterns). 

M. Patek, P. Safar, M. Smrcina, E. Wegrzyniak, K. Bjergarde, A. Weichsel, P. Strop, 2D and 3D spatially addressed arrays for high-throughput automated synthesis of combinatorial libraries. J. Comb. Chem. 2004, 6, 43-49. 

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