Sources and Care of Starter Cultures

Most lines are received as gifts from other scientists, who generally send a full bottle of cells through the mail. Actually, many repositories now favor shipping unfrozen cells. These cells when received, should simply be counted and diluted to about 5x10 cells/ml and placed in a 37°C, CO2 controlled incubator. 

Our second starter culture, line WI-L2, was a gift from Arthur Bloom, College of Physicians and Surgeons, Columbia University (New York, New 

Lymphoblastoid lines can be initiated from nearly any human donor, but discussion of this process is beyond the scope of our essay on mutation assay/  

Combine the lymphoblasts with the test agent and a suitable metabolizing element, and note the increase in the fraction of surviving cells that are able to form colonies under selective conditions. For readers who would prefer a more complete discussion, we next consider the mechanics of exposure, colony enumeration, selection procedures, and a number of idiosyncrasies of several types of mutants. 

We want to relate the observed experimental end point, the induced mutant fraction, to mutagen exposure. An appropriate expression for mutagen 

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