Somatic cell hybrid maps

Bennett, K. L., Lalley, P. A., Barth, R. K. and Hastie, N. D. (1982) Mapping the structural genes coding for the major urinary proteins in the mouse combined use of recombinant inbred strains and somatic cell hybrids. Proc. Natl. Acad. Sci. USA 79, 1220-4. 

The human A locus is located on chromosome 22. As in the case of the human Igh locus, the A genes were first tentatively mapped by studies measuring immunoglobulin gene expression in somatic cell hybrids [91] and the chromosome assignment later confirmed by direct hybridization studies [99]. 

A human gene in a somatic cell hybrid can also be detected and mapped without being expressed at the cellular level, e. g. by restriction enzyme digestion of DNA from the hybrid, followed by Southern blotting and detection of the human gene with a radioactively labeled probe, or by identifying primers which can be used in the Polymerase chain reaction (see) to amplify specifically the human and not the mouse gene. 

Ehrlich, G., Viegas-Pequignot, E., Ginzberg, D., Sindel, L., Soreq, H., Zakut, H. (1992). Mapping the human acetylcholinesterase gene to chromosome 7q22 by fluorescent in situ hybridization coupled with selective PCR amplification from a somatic hybrid cell panel and chromosome-sorted DNA libraries. Genomics 13 1192-7. 

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