Solid-phase peptide synthesis advantages

ELPs can be produced via chemical synthesis and biosynthetically. For chemical synthesis via solid phase peptide synthesis, the attainable polymer length is limited, and if long polymers with a defined length are required then the biosynthetic approach is more appropriate. An advantage of chemical synthesis is, however, that it enables the facile introduction of functional residues in the polypeptide [27]. 

Finally, dendrimers have been synthesized using solid phase peptide synthesis resins, wherein the core is linked to the resin and the half-dendrimer (dendron) is built out from it in sequential steps (Marsh et al., 1996 Swali et al., 1997 Wells et al., 1998). The advantage of this method... 

One advantage of the coenzyme amino acid chimera approach is that it is compatible with solid phase peptide synthesis. Consequently, the reactive functionality can be readily and selectively delivered to any site in the peptide. Additionally, both natural and unnatural residues can be incorporated throughout the peptide scaffold, and related compounds can be investigated rapidly by combinatorial synthesis techniques. 

Carpino, L. A., El-Faham, A., Minor, C. A., and Albericio, E. (1994) Advantageous applications of azabenzotriazole (triazolopyridine)-based coupling reagents to solid-phase peptide synthesis J. Chem. Soc. Chem. Commun. 2, 201-204. 

Another method to introduce unnatural amino acids into a polypeptide chain is through complete chemical synthesis (10). The predominantly used method, stepwise solid-phase peptide synthesis (SPPS), attaches the C-terminal amino acid to a solid support, and amino acids are added one at a time to the N-terminus. A clear advantage of chemical synthesis is that it enables the accurate introduction of unnatural amino acids at any site in a protein. The number of unnatural amino acids that can be introduced is limited only to the size of the chain, and chains of entirely unnatural amino acids can be produced using this method. Chemical synthesis is useful particularly for the incorporation of isotopic labels and unnatural amino acids that are toxic to cells or incompatible with the translational machinery. However, construction of a polypeptide chain using even the most advanced chemical synthesis techniques is daunting when confronted with the construction of an entire protein, as these methods currently are limited to approximately 100 amino acids (10). 

One advantage of this method over solid phase peptide synthesis is the possibility offered by homogenous conditions to perform convergent supported synthesis. In this case, two peptides, one synthesized by the direct approach and the other synthesized through the reverse strategy, can be coupled to each other as they... 

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