Peptide synthesis advantages

ELPs can be produced via chemical synthesis and biosynthetically. For chemical synthesis via solid phase peptide synthesis, the attainable polymer length is limited, and if long polymers with a defined length are required then the biosynthetic approach is more appropriate. An advantage of chemical synthesis is, however, that it enables the facile introduction of functional residues in the polypeptide [27]. 

Reversed micelles have also shown to be useful not only in bioconversions, but also in organic synthesis. Shield et al. (1986) have reviewed this subject and brought out its advantages in peptide synthesis, oxidation or reduction of steroids, selective oxidation of isomeric mixtures of aromatics, etc. In the oxidation of aromatic aldehydes to carboxylic acids with enzymes hosted in reverse micelles, the ortho substituted substrates react much more slowly than other isomers. 

The role of reversed micelles in the manufacture of fine chemicals with enzymes also needs to be assessed and analysed. An outstanding example is lipase catalysed interesterification to produce cocoa butter substitute from readily available cheap materials (Luisi, 1985). This example of reversed micelles is sometimes referred to as a colloidal solution of water in organic systems. A number of water insoluble alkaloids, prostanoids, and steroids have been subjected to useful transformations (Martinek et al., 1987). Peptide synthesis has also been conducted. The advantages of two liquid phases are retained to a very great extent the amount of water can be manipulated to gain advantages from an equilibrium viewpoint. 

Finally, dendrimers have been synthesized using solid phase peptide synthesis resins, wherein the core is linked to the resin and the half-dendrimer (dendron) is built out from it in sequential steps (Marsh et al., 1996 Swali et al., 1997 Wells et al., 1998). The advantage of this method... 

C-terminal deprotection was performed using morpholine as an allyl trap, resulting in high yields. The most significant advantage of the allyl group in this peptide synthesis in comparison with the choline ester is that it overcomes the solubility problem encountered in the enzymatic deprotection approach. The deprotection of the allyl ester is performed in organic solvents, in which these lipidated peptide are readily soluble. ... 

One advantage of the coenzyme amino acid chimera approach is that it is compatible with solid phase peptide synthesis. Consequently, the reactive functionality can be readily and selectively delivered to any site in the peptide. Additionally, both natural and unnatural residues can be incorporated throughout the peptide scaffold, and related compounds can be investigated rapidly by combinatorial synthesis techniques. 

Sequencing by both Edman sequence analysis and mass spectrometric analysis can be complimentary and where one may fail in yielding information concerning the synthesis problem, the other may succeed. This is because every peptide has different properties and each technique has advantages and disadvantages. However, sequence analysis is expensive and time consuming, which must be taken into consideration when solving the problems of peptide synthesis, especially those of routine peptide synthesis. 

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