Sodium Nitroferricyanide

Sodium nitroprusside (for sulfur detection) dissolve about 1 g of sodium nitroprusside in 10 mL of water as the solution deteriorates on standing, only freshly prepared solutions should be used. This compound is also called sodium nitroferricyanide and has the formula Na2pe(NO)(CN)5 2H2O. 

For the nitroprusside-cyanide reagent two solutions are prepared. For solution I, sodium nitroferricyanide (sodium nitroprusside, 1.5 g) is dissolved in 2M H2S04 (5mL) to which MeOH (95 mL) and NH4OH (10 mL) is added. A precipitate forms that is discarded. The clear, red solution is refrigerated. For solution II, sodium cyanide (2g) is dissolved in H20 (5 mL) and diluted with MeOH (95 mL), this is filtered if necessary. CAUTION Sodium cyanide can be absorbed through the skin and is extremely toxic. Appropriate safety precautions and first aid procedures should be adopted when handling sodium cyanide. 

A. Add a few drops of sodium nitroferricyanide TS to 2 mL of a 1 50 aqueous solution, then add 1 mL of approximately 0.1 N sodium hydroxide. A transient blue color immediately appears. 

When solutions of lactates are acidified with sulfuric acid, potassium permanganate TS (0.1 AO is added, and the mixture is heated, acetaldehyde is evolved. This can be detected by allowing the vapor to come into contact with a filter paper that has been moistened with a freshly prepared mixture of equal volumes of 20% aqueous morpholine and sodium nitroferricyanide TS. A blue color is produced. 

Sodium Nitroferricyanide TS Dissolve 1 g of sodium ni-troferricyanide [Na2Fe(N0)(CN)5-2H20] in water to make 20 mL. Prepare this solution fresh. 

Acetaldehyde Test Paper Use a solution prepared by mixing equal volumes of a 20% solution of morpholine and a 5% solution of sodium nitroferricyanide. Saturate the prepared filter paper in the mixture, and use the moistened paper without drying. 

Synonym Sodium nitroferricyanide Trade name Nitropress (Hospira)... 

POase activity in tissue or cell preparations is usually eliminated prior to the test. In some cases, endogenous POase is confined to peroxisomes and does not interfere with the test. Fixation in methanol is often accompanied by a decrease in peroxidatic activity. The method of Straus (1971) using 1% sodium nitroferricyanide and 1% acetic acid in methanol, is effective to abolish endogenous POase, but also weakens the immunohistochemical reaction. Streefkerk (1972)... 

Sodium nitroferricyanide Sodium nitrosylpentacyanoferrate (III) dihydrate B.P., U.S.P. Nipride (Hofiinann-La Roche) Nitropress (Abbott). 

Sodium Nitroprusside (Sodium Nitroferricyanide Nitropress Nipride)... 

Sulfur The original solution is reacted with an approximately 1 % aqueous sodium nitroferricyanide solution. A deep violet color indicates sulfur. This reaction is very sensitive to confirm, add a drop of the alkaline solution of the sample under investigation to a silver coin. If sulfur is present, a brown spot of silver sulfide will form. An alternative procedure is to acidify the original solution with acetic acid (test with litmus or pH paper) and then add several drops of aqueous 2 M lead acetate solution or test with lead acetate paper. A black precipitate of lead sulfide or darkening of the paper indicates sulfur. 

Sodium nitropmsside (reagent for hydrogen sulfide and wool). Use a fi eshly prepared solution of 1 g of sodium nitroferricyanide in 10 mL of water. 

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