Sodium dodecyl sulphate SDS

Two-dimensional gel electrophoresis (2DE) is a two-dimensional technique for protein separation, which combines isoelectric focusing and sodium dodecyl sulphate (SDS) electrophoresis. The high resolving power results from separation according to charge (isoelectric point) in the first dimension and size (mobility in a porous gel) in the second dimension. Depending on the gel size, from several hundred to more than 5,000 proteins can be separated. 

Homogeneous LaMn03 nanopowder with the size of 19-55 nm and with the specific surface area of 17-22 m2/g has been synthesized using a surfactant, sodium dodecyl sulphate (SDS) to prevent agglomeration [47], The sonochemically prepared LaMn03 showed a lower phase transformation temperature of 700°C, as compared to the LaMn03 prepared by other conventional methods which has been attributed to the homogenization caused by sonication. Also, a sintered density of 97% of the powders was achieved for the sonochemically prepared powders at low temperature than that of conventionally prepared powders. 

Fig. 15.5 Long-exposure photographs recorded for argon-saturated water (a) and luminol solution (b) and (c) 10 mM sodium dodecyl sulphate (SDS) solution. The emission spectra in (d) are from pure argon-saturated water and 10 mM SDS solution. Note the sodium D line at 589 nm. In this experiment, sonication was performed at 159 kHz...

Modified CNTs feature various spectral changes depending on the methods and the location of modifications. These changes include variations in band frequencies, width, and intensities. For example, aryldiazonium salts [139] were used to modify individual sodium dodecyl sulphate (SDS) coated SWNTs with aryl group. The Raman spectrum of functionalized (SDS-free) SWNTs shows a disorder mode much higher than pristine SWNT the radial breathing modes are nearly unobservable. 

HTAC cationic micelles also markedly enhance the CL intensity of fluorescein (FL) in the oxidation of hydrogen peroxide catalyzed by horseradish peroxidase (HRP) [39], However, no CL enhancement was observed when anionic micelles of sodium dodecyl sulphate (SDS) or nonionic micelles of polyoxyethylene (23) dodecanol (Brij-35) were used (Fig. 9). CL enhancement is attributed to the electrostatic interaction of the anionic fluorescein with the HTAC micelles. The local concentration of fluorescein on the surface of the micelle increases the efficiency of the energy transferred from the singlet oxygen (which is produced in the peroxidation catalyzed by the HRP) to fluorescein. This chemiluminescent enhancement was applied to the determination of traces of hydrogen peroxide. The detection limit was three times smaller than that obtained in aqueous solution. 

Figure 10.12 Micelles of sodium dodecyl sulphate (SDS) comprise as many as 80 monomer units. The micelle interior comprises the hydrocarbon chains, and is oil like. The periphery presented to the water of solution is made up of hydrated hydrophilic sulphonic acid groups...

PAG electrophoresis is an important technique in many areas. In DNA sequencing it provides a fundamental method of separation, and in protein studies it is important both in the form described here and also in conjunction with the detergent, sodium dodecyl sulphate (SDS), giving a valuable method for assessing the relative molecular mass of a protein. 

Sodium dodecyl sulphate (SDS), purest grade, C 2H2sNa04S harmful... 

Surfactants such as cetyl trimethyl ammonium bromide (CTAB), Triton X-100 (TX-lOO) and sodium dodecyl sulphate (SDS) are the most commonly used. CTAB forms large micelles [24-26] with aggregation number 61, cmc 9.2 X 10 M, and a positive micellar Stem layer TX-lOO has aggregation number 139 with neutral OH groups on the Stern layer, and SDS forms negative micelles with cmc 8.3 x 10 M and aggregation number 131. The... 

Make two identical surfactant solutions by dissolving 2.5 g sodium dodecyl sulphate (SDS) in lO.Ocm of distilled water at room temperature. (The solution may need to be heated to dissolve the surfactant.) Take care excessive shaking will cause foaming. 

A molecularly imprinted polypyrrole film coating a quartz resonator of a QCM transducer was used for determination of sodium dodecyl sulphate (SDS) [147], Preparation of this film involved galvanostatic polymerization of pyrrole, in the presence of SDS, on the platinum-film-sputtered electrode of a quartz resonator. Typically, a 1-mA current was passed for 1 min through the solution, which was 0.1 mM in pyrrole, 1 mM in SDS and 0.1 M in the TRIS buffer (pH = 9.0). A carbon rod and the Pt-film electrode was used as the cathode and anode, respectively. The SDS template was then removed by rinsing the MlP-film coated Pt electrode with water. The chemosensor response was measured in a differential flow mode, at a flow rate of 1.2 mL min-1, with the TRIS buffer (pH = 9.0) as the reference solution. This response was affected by electropolymerization parameters, such as solution pH, electropolymerization time and monomer concentration. Apparently, electropolymerization of pyrrole at pH = 9.0 resulted in an MIP film featuring high sensitivity of 283.78 Hz per log(conc.) and a very wide linear concentration range of 10 pM to 0.1 mM SDS. 

The solubility of proteins from parasitic organisms can often be enhanced by the use of hot sodium dodecyl sulphate (SDS) before solubilization in SD buffers. The ability of SDS to denature proteins also helps in allowing access to hydrophobic proteins, not normally seen after standard preparation procedures. SDS treatment in whole F. hepatica preparations has been shown to yield more protein spots visualized on gel analysis than other methods (Jefferies et a/., 2000). 

A small area of the outer surface of the upper arm is exposed to test and control materials under occlusive cover. The test and control materials are applied to round chambers and taped to the arm with surgical tape. A standard irritant (20% sodium dodecyl sulphate [SDS]) is used as the positive control. Each panellist has up to four patches applied, each patch being applied for an increased duration. Due to the potential irritancy of the test materials a cautious approach is used. The first patch is applied for one hour, the second, third, and fourth patches for two, three, and four hours, respectively. Each patch is applied to a different site on consecutive weeks. This approach allows any unexpected or unacceptable reactions to be limited to a minimum. 

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