Sodium azide reagent

Several wet chemical tests have been used to identify the presence of sulphur in plastics including rubbers (Verleye et al., 2001). A quick test to detect the presence of sulphur is based on a sodium azide reagent (Daniels and Ward, 1982). The test is based on the catalytic decomposition of a sodium azide/iodine solution by sulphur-containing groups resulting in the evolution of nitrogen bubbles ... 

A. Iodine-sodium azide reagent in combination with starch soln. produces white spots on a blue-gray background, but they are labile and have to be photographed... 

Azide ion ( N=N=N ) Sodium azide IS a reagent used for carbon-nitrogen bond formation The product IS an alkyl azide... 

Alkyl azides prepared by nucleophilic substitution of alkyl halides by sodium azide as shown m the first entry of Table 22 3 are reduced to alkylammes by a variety of reagents including lithium aluminum hydride... 

The nucleophilic attack of nitrogen bases leads to a variety of products as the result of addition or addition-elimination reactions The regioselectivity resembles that of attack by alcohols and alkoxides an intermediate carbanion is believed to be involved In the absence of protic reagents, the fluorocarbanion generated by the addition of sodium azide to polyfluonnated olefins can be captured by carbon dioxide or esters of fluonnated acids [J 2, 3] (equation I)... 

Another nittogen-containing inflate derivative, trifluoromethanesuifonyi azide, IS prepared by the reaction of triflic anhydride with aqueous sodium azide [775] and IS used as an efficient reagent for the synthesis of alkylazides from alkylanunes (equation 58)... 

The polymer (SN)x may also be made in a two-step process using azide reagents. Thus the reaction of S2CI2 with sodium azide in... 

The use of azide reagents is also important for the synthesis of cyclic sulfur(VI)-nitrogen systems. The reaction of SOCI2 with sodium azide in acetonitrile at -35°C provides a convenient preparation of the trimeric sulfanuric chloride [NS(0)C1]3 (Eq. 2.16). " Thionyl azide, SO(N3)2 is generated by the heterogeneous reaction of thionyl chloride vapour with silver azide (Eq. 2.17). This thermally unstable gas was characterized in situ by photoelectron spectroscopy. The phenyl derivative of the six-membered ring [NS(0)Ph]3 can be prepared from lithium azide and PhS(0)Cl. ... 

The Schmidt reaction of ketones works best with aliphatic and alicyclic ketones alkyl aryl ketones and diaryl ketones are considerably less reactive. The reaction is only seldom applied to aldehydes as starting materials. The hydrazoic acid used as reagent is usually prepared in situ by treatment of sodium azide with sulfuric acid. Hydrazoic acid is highly toxic, and can detonate upon contact with hot laboratory equipment. 

In a 1-1. three-nccked, round-bottomed flask equipped with a mechanical stirrer, reflux condenser, and powder funnel are placed 180 ml. of reagent grade chloroform, 16.0 g. (0.16 mole) of cyclobutanecarboxylic acid (Note 1), and 48 ml. of concentrated sulfuric acid. The flask is heated in an oil bath to 45-50°, and 20.0 g. (0.31 mole) of sodium azide (Note 2) is added over a period of 1.5 hours (Note 3). After the addition of sodium azide is complete, the reaction mixture is heated at 50° for 1.5 hours. The flask is cooled in an ice bath, and approximately 200 g. of crushed ice is added slowly. A solution of 100 g. of sodium hydroxide in 200 ml. of water is prepared, cooled to room temperature, and then added slowly to the reaction mixture until the pH of the mixture is approximately 12—13. The mixture is poured into a 2-1. three-necked, round-bottomed flask, the... 

Iodine-Potassium Iodide Solution-Sodium Azide-Starch Reagent... 

This reaction is catalyzed by the presence of divalent sulfur — e.g. sulfur ions, substances with S-S links, thioethers, thiazoles. If such substances are present in a chromatogram zone then the iodine applied with the reagent is consumed by reaction with sodium azide according to the above reaction. It is no longer available for the for-... 

Analytical reagent-grade sodium azide purchased from Alfa Division, Ventron Corporation, or J. T. Baker Chemical Company was used as supplied. 

Ross, F. F., Water Waste Treatment, 1964, 9, 528 private comm., 1966 One of the reagents required for the determination of dissolved oxygen in polluted water is a solution of sodium azide in 50% sulfuric acid. It is important that the diluted acid should be quite cold before adding the azide, since hydrogen azide boils at 36°C and is explosive in the condensed liquid state. 

The Schmidt reaction is also applied to a variety of 1-tetralones, yielding (51). The use of sodium azide in acetic acid and sulphuric acid [7, 12, 13, 30, 34, 36, 37, 72, 73, 84] is preferable to the procedure in the earlier stage, in which hydrazoic acid, sulphuric acid and chloroform are employed [115]. Other acidic reagents such as polyphosphoric acid [116, 117], sulphuric acid [116, 118], methasulphonic acid [119] and trichloroacetic acid [116] are used in some cases. Variation of substituents affects the course of the rearrangement 6-methoxytetralones are rather liable to afford the isomeric 2-benzazepine-l-ones in preference to the desired (51) [ 7, 116, 118]. The Schmidt reaction is also conveniently applied to various 1,4-naphthoquinones and yields a wide range of 2,5-dihydro-l-benzazepin-2,5-diones [85, 120-122]. 

Notes. When using biotin-labeled secondary antibodies instead of enzyme-labeled antibodies, you have first to detect biotin with enzyme-labeled (strept) avidin and proceed further with the Substrate Step (9). Do not add normal serum, non-fat dried milk, culture media or other potential sources of biotin to (strept)avidin-containing reagents. This may result in reduced sensitivity. Solutions containing sodium azide or other inhibitors of peroxidase activity should not be used in diluting the peroxidase substrate. 

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