Small intestine brush border

Di- and tripeptides Aminopeptidases Hydrolyze di- and tripeptides into amino acids Absorptive cells of small intestine Brush border of absorptive cells... 

Poschet, J. F., S. M. Hammond, and P. D. Fairclough. Characterisation of penicillin-G uptake in rabbit small-intestinal brush-border membrane vesicles. Biochim. Biophys. Acta 1996, 1278, 233-240. 

Kitagawa, S., J. Takeda, and S. Sato. pH-dependent inhibitory effects of angiotensin-converting enzyme inhibitors on cefroxadine uptake by rabbit small intestinal brush-border membrane vesicles and their relationship with hydrophobicity and the ratio of zwitterionic species. Biol. Pharm. Bull. 1999, 22, 721-724. 

Hashimoto, N., et al. Renin inhibitor transport mechanism in rat small intestinal brush-border membrane vesicles. Pharm. Res. 1994, 11, 1448— 1451. 

Thurnhofer, H. and Hauser, H. (1990) Uptake of cholesterol by small intestinal brush border membrane is protein-mediated. Biochemistry 29, 2142-2148. 

Murer H, Gmaj P, Steiger B, et al. Transport studies with renal proximal tubular and small intestinal brush border and basolateral membrane vesicles vesicle heterogeneity, coexistence of transport system. Methods Enzymol 1989 172 346-364. 

Semenza, G., Kessler, M Hosang, M Weber, J., Schmidt, U. (1984). Biochemistry of the Na+, D-glucose cotransporter of the small intestinal brush-border membrane. The state of the art in 1984. Biochim. Biophys. Acta 779,343-379. 

Bolte G, Seilmeier W, Wieser H, Holm K, Beuermann K, Newport B, et al. Enhanced peptide-binding capacities of small intestinal brush border membranes in celiac disease. Pediatr Res 1999 46 666-670. 

Malo C and Wilson JX (2000) Glucose modulates vitamin C transport in adult human small intestinal brush border membrane vesicles. Journal of Nutrition 130,63-9. 

Schulthess, G-, sird Hauser, H. (1995). A unique feature of lipid dynamics in small intestinal brush border membrane. Mol. Membr. Biol, 12,1U5-112. 

Kessler, M., O. Acuto, C. Storelli, H. Murer, M. Muller, and G. Semenza. 1978. A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Biochim. Biophys. Acta 506 136-154. 

The most widely used ceU line in drug transport studies is the Caco-2 cell line. This is an iimnortal ceU line derived from human colon carcinoma that can be grown to monolayer on porous support. Functionally, this cell line models the colon more than the small intestine. The Caco-2 model allows characterization of both mucosal-to-serosal and serosal-to-mucosal transport and can also be used to study transcellular and paracellular transport, hi addition to expressing small intestinal brush border enzymes, Caco-2 cells also express Phase I and Phase II enzymes and can be used to evaluate metabohsm of compounds during transport across the intestinal barrier. ... 

Some recent studies on vitamin transport using membrane vesicles include those of vitamin B6 by rat kidney brush border membranes (Bowman et al, 1990), ascorbic acid by teleost intestinal brush border membranes (Mafha et ai, 1993), biotin by human kidney brush border membranes (Baur and Baumgartner, 1992), pantothenate by human placental brush border membranes (Grassl, 1992), folate and riboflavin by rabbit intestinal brush border membranes (Said and Mohammadkhani, 1993a,b Said et al, 1993), and thiamine by rat small intestine basolateral membranes (Laforenza et al, 1993). Bile acid transport in human placental, rat ileal, and rabbit small intestinal brush border membrane vesicles (Dumaswala et al, 1993 Gong et al, 1991 Kramer et al, 1993) and the effect of vitamin D status... 

Dudeja et al. (1991), using rat small intestine brush border membranes treated with benzyl alcohol. 

Dudeja, P. K., Wali, R. K., Harig, J. M and Brasitus, T. A. (1991). Characterization and modulation of rat small intestine brush-border membrane transbilayer fluidity. Am. J. Physiol. 260, G586. 

Hauser, H., Howell, K., Dawson, R. M. C., and Bowyer, D. E. (1980). Rabbit small intestinal brush border membrane preparation and lipid composition. Biochim. Biophys. Acta 602, 567. 

It is now generally accepted that the digestion of starch by many mammalian species involves, firstly, the action of pancreatic oc-amylase to give oligosaccharide end-products which are hydrolysed further to D-glucose by the enzymes of the small intestine brush border. The enzymology of these reactions has been reviewed" with respect to both nutritional and clinical implications. 

Kitagawa, S. Takeda, J. Sato, S. Uptake of enalapril by rabbit small intestinal brush-border membrsine vesicles, Biol.Pharm.BulL, 1999, 22, 762-764. 

The kinetic properties and substrate specificities of known glycoside hydrolases have been tabulated and indexed. The oligosaccharidases of small-intestinal brush borders have been reviewed. ... 

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