Membrane vesicle intestinal basolateral

INTESTINE Characterization of a membrane potassium ion conductance in intestinal secretory cells using whole cell patch-clamp and calcium-sensitive dye techniques, 192, 309 isolation of intestinal epithelial cells and evaluation of transport functions, 192, 324 isolation of enterocyte membranes, 192, 341 established intestinal cell lines as model systems for electrolyte transport studies, 192, 354 sodium chloride transport pathways in intestinal membrane vesicles, 192, 389 advantages and limitations of vesicles for the characterization and the kinetic analysis of transport systems, 192, 409 isolation and reconstitution of the sodium-de-pendent glucose transporter, 192, 438 calcium transport by intestinal epithelial cell basolateral membrane, 192, 448 electrical measurements in large intestine (including cecum, colon, rectum), 192, 459... 

Absorption Epithelial cells Blood Everted sac, Ussing-chamber experiments using intestinal epithelium, basolateral membrane vesicles, Caco-2 cells monolayer... 

Murer H, Gmaj P, Steiger B, et al. Transport studies with renal proximal tubular and small intestinal brush border and basolateral membrane vesicles vesicle heterogeneity, coexistence of transport system. Methods Enzymol 1989 172 346-364. 

Shoji T, Suzuki H, Kusuhara H, et al. ATP-dependent transport of organic anions into isolated basolateral membrane vesicles from rat intestine. Am J Physiol Gastrointest Liver Physiol 2004 287 G749-G756. 

Some thiamin is phosphorylated to thiamin monophosphate in the intestinal mucosa, although this is not essential for uptake, and isolated membrane vesicles wUl accumulate free thiamin against a concentration gradient. Thiamin does not accumulate in the mucosal cells there is sodium-dependent active transport across the basolateral membrane, so that the mucosal concentration of thiamin is lower than that in the serosal fluid (Hindi et al., 1984 Hindi and Laforenza, 2000 Dudeja et al., 2001). 

Although only CaBP mRNA is known to increase in response to vitamin D, other vitamin D-dependent changes occur in the intestinal epithelium, including increases in activity of alkaline phosphatase, calcium ATPase, adenylate cyclase, and RNA polymerase. In response to vitamin D, several brush-border membrane proteins increase in concentration as does a calcium-binding complex. Non-cAMP-dependent phosphorylation of a brush-border membrane protein, increased synthesis and turnover of microvillar membrane phospholipids, and effects on mitochondria, Golgi membranes, and intracellular membrane vesicles are observed. Increased transport of Ca " " across the basolateral membrane may be produced by... 

Some recent studies on vitamin transport using membrane vesicles include those of vitamin B6 by rat kidney brush border membranes (Bowman et al, 1990), ascorbic acid by teleost intestinal brush border membranes (Mafha et ai, 1993), biotin by human kidney brush border membranes (Baur and Baumgartner, 1992), pantothenate by human placental brush border membranes (Grassl, 1992), folate and riboflavin by rabbit intestinal brush border membranes (Said and Mohammadkhani, 1993a,b Said et al, 1993), and thiamine by rat small intestine basolateral membranes (Laforenza et al, 1993). Bile acid transport in human placental, rat ileal, and rabbit small intestinal brush border membrane vesicles (Dumaswala et al, 1993 Gong et al, 1991 Kramer et al, 1993) and the effect of vitamin D status... 

Laforenza, U. L., Gastaldi, G., and Hindi, G. (1993). Thiamine outflow from the enterocyte A study using basolateral membrane vesicles from rat small intestine. /. Physiol. (London) 468,401. 

Figure 4 Transcellular transport in the proximal and distal intestine of the suckling rat. (A) In the proximal intestine, macromolecules may be selectively absorbed in coated pits along luminal membranes (M) and transported via coated vesicles to the basolateral surface (arrows). Alternatively, selection may occur in tubular compartments (T) or from endosomal vesicles (V). (B) In the distal intestine selective absorption may occur in coated pits at the luminal membrane (M) or from the numerous tubules (T) of the elaborate endocytic complex. (From Ref. 18.)...

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