Size exclusion chromatograph light scattering detector

Upnmoor and Brunner [7] evaluated a packed column SFC with a light scattering detector using a mobile phase of carbon dioxide modified with methanol for the chromatography of polymer additives. Moulder and co-workers [8] transferred eluent fractions from a packed capillary size exclusion chromatographic (SEC) column using a solvent vented interface to an open tubular CSFC column. The system was evaluated for the analysis of polymer additives. 

The most common detector is a differential refractometer, which then is proportional to the concentration of polymer at that elution volume. More-sophisticated detectors include a low-angle, light-scattering device that, in tandem with the differential refractometer, yields a direct measme of molecular weight associated with particles at the observed elution volume. Size-exclusion chromatographs are now highly engineered instruments that have proven invaluable to research on macromolecules. 

As mentioned in Chap. 1, randomly hyperbranched chains are even more complicated than dendrimers. It has not been completely clear whether they are fractal objects [11, 12] and whether those previously reported M-dependent intrinsic viscosities from an on-line combination of the size exclusion chromatograph (SEC) with viscosity and multi-angle laser light scattering (MALLS) detectors actually captured its structure-property relationship [11, 13]. In the next section, we ll discuss our experimental results in detail. 

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