SiRNA technology

Small Interfering RNA (siRNA) Technology. siRNA, a class of 20-25 nucleotide-long RNA molecules, is used in RNA silencing, a method that allows one to knock down expression of genes or more specifically to degrade the target mRNAs, in a sequence-specific fashion (Chakraborty 2007 Kumar... 

Cocks, B. G., and T. P. Theriault. 2003. Developments in effective application of small inhibitory RNA (siRNA) technology in mammalian cells. Drug Discov Today Targets 3 165-71. 

Before the advent of gene overexpression methods, small interfering RNA (siRNA) technologies, and other procedures to produce genetic knockout systems, natural products, and other biologically active small molecule-based compounds provided the first efficient means to probe cell biology. Natural toxins and... 

Rat thyroid cell lines have been much in use. They have also lost all structural aspects and functions of the thyroid except transport. In spite of differences in signal transduction, they are useful to study the effects of hormones, oncogenes, specific proteins by siRNA technology, and so on, provided they are validated for the metabolism studied. PCC13 cell line is considered as the choice material (Kimura et al., 2001). Some iodide effects have been observed at very high iodide concentrations (lOmM), but as explained above they are not considered here. It is interesting to note that when reconstituted as follicles in vivo after transplantation, the same cells are 200 times more sensitive to iodide (Aeschimann et al., 1994). In this review, we only consider the effects observed with suitable models at iodide concentrations comparable to those obtained in vivo. 

Fuchs U, Borkhardt A. The application of siRNA technology to cancer biology discovery. 

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