Single cell electropherograms

First Label-free Single Cell Electropherograms. 316... 

Figure 12. (A) Baseline corrected electropherogram from a single Sf9 cell with native UV-LIF detection at a separation voltage of 240 V/cm. Peak groups I, II and III (dotted ellipses) correspond to 65, 90, and 130 s, respectively. (B) Electropherogram of four St9 cells at a separation voltage of 360 V/cm. Peak groups I, II and III (dotted ellipses) correspond to 37, 74, and 87 s, respectively. (C) Baseline corrected single cell electropherogram of St9 cell with 124 peaks with native UV-LIF detection.

FIGURE 14.8 Electropherogram of an a-glucosidase II single-cell (Cell 7) reaction aliquot taken after incubation (a). Percent conversion of the dissaccharide (DG-TMR) substrate by a-glucosidase II in single cells and control assays (b). Percent conversion was calculated by comparing the DG-TMR peak to that of the monosaccharide product MG-TMR. (From Shoemaker, GK. et al.. Anal. Cheni., 77, 3132, 2005. With permission.)... 

Figure 12.10. (a) Electrokinetic microinjector and (b) electropherogram generated from the removal and separation of cytoplasm samples from single nerve cells of Planorbis corneus, a pond snail.8 [Reprinted, by permission, from R. A. Wallingford and A. G. Ewing, Anal. Chem. 60 (No. 18), 1988, 1972-1975. Capillary Zone Electrophoresis with Electrochemical Detection in 12.7 pm Diameter Columns . 1988 by American Chemical Society.]... 

FIGURE 14.3 Electropherogram of the separated contents from a single dopaminergic neuron obtained using CE with scanning electrochemical detection and plotted in three dimensions. Peaks a and b correspond to dopamine that was immediately released during lysis and dopamine located deeper in the cell, respectively. Peak c represents a neutral electroactive species in cellular buffer. (From Swanek, F.D. et al., Anal. Chem., 68, 3912, 1996. With permission.)... 

FIGURE 14.7 Electropherogram of multiplex RT-PCR products correspoudiug to P-actin and the a estrogen receptor from single human breast carcinoma cells. (From Zabzdyr, J.L. and LiUard, S.J., Electrophoresis, 26, 137, 2005. With permission.)... 

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