Silver nanoparticles fluorescence enhancement

Corrigan, T, Guo, S., Phaneuf, R., and Szmacinski, H. (2005) Enhanced fluorescence from periodic arrays of silver nanoparticles,/. Fluoresc., 15, 777-784. 

The commercially available dicyanomethylene squaraine dye Seta-670-mono-NHS showed extremely low blinking effects and good photostability when used in single-molecule studies of multiple-fluorophore labeled antibodies [113]. Seta-670-mono-NHS and Seta-635-NH-mono-NHS were covalently labeled to antibodies and used in a surface-enhanced immunoassay [114]. From the fluorescence intensity and lifetime changes determined for a surface that had been coated with silver nanoparticles, both labeled compounds exhibited a 15- to 20-fold... 

Sprensen TJ, Laursen BW, Luchowski R, Shtoyko T, Akopova I, Gryczynski Z, Gryczynski I (2009) Enhanced fluorescence emission of Me-ADOTA+ by self-assembled silver nanoparticles on a gold film. Chem Phys Lett 476 46-50... 

Figure 27.1 Principle of silver nanoparticle enhanced fluorescence applied to artificial cell surfaces.

Lochner, N., Pittner, F., Wirth, M., Gabor, F., Wheat germ agglutinin binds to the epidermal growth factor-receptor of artificial Caco-2 membranes as detected by silver nanoparticle enhanced fluorescence. Pharm Res 20, 833-839 (2003). 

The mechanistic route followed for the reduction process was different in the case of Ag/mycelium and Ag/media. In media, glucose was found mainly responsible for the reduction whereas in the case of mycelium, it was mainly the S-H group responsible for the same. The photoluminescence spectrum of these protein-stabilized silver nanoparticles also showed much enhanced fluorescence emission intensity. 

K. Aslan, P. Holley, and C. D. Geddes. Metal-enhanced fluorescence from silver nanoparticle-deposited polycarbonate substrates Journal of Materials Chemistry, 2006, 16, 2846-2852. 

Zhang, J., Malicka, J., Gryczynski, I., and Lakowicz, J. R. (2005). Surface-enhanced fluorescence of fluorescein-labeled oligonucleotides capped on silver nanoparticles, yowrno/ of Physical Chemistry B 109 7643-7648. 

Sabanayagam, C.R. and Lakowicz, J.R. (2007) Increasing the sensitivity of DNA microarrays by metal-enhanced fluorescence using surface-boimd silver nanoparticles. Nucleic Acids Res 35 el3. 

Figure 7.1 (A) Sdiematic representation of the Metal-Enhanced Fluorescence phenomena (B) FDTD calculations for two silver nanoparticle arrays...

Both Geddes and the Lakowicz group s have investigated the metal-enhanced fluorescence of fluorophores on silver island films (SIFs) [11,26,27] and variously aggregated silver nanoparticles in solution [28,29]. One example of enhancement on SIFs is discussed below [26]. In this work the distance-dependent MEF of a monolayer of sulforfiodamine B (SRB) on SIFs was studied. A SRB monolayer was electrostatically incorporated into the Langmuir-Blodgett (LB) layers of octadecylamine (ODA) deposited... 

Metal nanoparticles have attracted considerable interest due to their properties and applications related to size effects, which can be appropriately studied in the framework of nanophotonics [1]. Metal nanoparticles such as silver, gold and copper can scatter light elastically with remarkable efficiency because of a collective resonance of the conduction electrons in the metal (i.e., the Dipole Plasmon Resonance or Localized Surface Plasmon Resonance). Plasmonics is quickly becoming a dominant science-based technology for the twenty-first century, with enormous potential in the fields of optical computing, novel optical devices, and more recently, biological and medical research [2]. In particular, silver nanoparticles have attracted particular interest due to their applications in fluorescence enhancement [3-5]. 

Rativa, D., Gomes, A. S. L., Wachsmann-Hogiu, S., Farkas, D. L., and de Araujo, R. E. (2008). Nonlinear Excitation of Tryptophan Emission Enhanced by Silver Nanoparticles. J. Fluoresc, Online. 

Figure 19.6 Experimental geometries for distance dependent control of fluorescence enhancement on top of silver nanoparhcles (structure I) and underneath silver nanoparticles (structure II), respectively. Re vinted from reference 48 with permission of the SPIE.

Figure 1.7. (A) Graphical representation of our laboratory s current interpretation of Metal-Enhanced S2 emission (Bottom). IC-Intemal Conversion, VR-Vibrational energy relaxation. Ag-Silver nanoparticle (SIFs), TCP-Transfer/coupling to Plasmons, MES2 -Metal Enhanced S2 Emission. Energy level spacing not drawn to scale. Fluorescence emission spectra, lex = 338 nm, of Azulene sandwiched between two SiFs and unsilvered slides at room temperature (B) Room TeiTq)erature, RT and (C) at 77K.

---