Silver enhanced immunogold

B. brassicae tissue fixed with glutaraldehyde was visualized by light microscopy using silver enhanced immunogold localization and showed the presence of myrosinase over the skeletal muscle of the head, thorax, and abdomen. Labelling was restricted to skeletal muscle, while in all sections examined flight muscles remain free of myrosinase. 

Silver-Enhancement Immunogold staining can be used successfully at the light micro-for Light Microscopy scopic level if the gold is silver-enhanced. Enhancing solutions... 

Combined neurotracing and immunolabeling can also be applied for electron microscopic analysis. We usually visualize BDA-labeled CFs with immunoperoxidase and DAB, and molecular markers with pre-embedding silver-enhanced immunogold. With this technique, CFs of different neuronal origins are differentially... 

Goode NP, Shires M, Crellin DM, et al. Post-embedding double-labeling of antigen-retrieved ultrathin sections using a silver enhancement-controlled sequential immunogold (SECSI) technique. J. Histochem. Cytochem. 2004 52 141-144. 

Fig. 1. Schematic drawing of various immune methods for localization of antigens. (A) Direct method. (B) Indirect method. (C) Immunogold with silver enhancement. (D) Peroxidase-antiperoxidase method. (E) Avidin-biotin system.

Yamashita S. Intranuclear localization of hormone-occupied and -unoccupied estrogen receptors in the mouse uterus application 1 nm immunogold-silver enhancement procedure to ultrathin frozen sections. JElectron Microsc 1995 44 22-29. 

Key Words Immunocytochemistry immunogold silver enhancement immunofluorescence immunoenzyme avidin biotin IGSS PAP APAAP epipolarization. 

Common chromogen systems currently in use include diaminobenzidine (DAB), 3-amino-9-ethyl-carbazole (AEC), Hanker-Yates reagent, alpha-naphthol pyronin used with peroxidase as substrate fast blue, fast red, BCIP- (5-bromo-4-chloro-3-indolyl phosphate) NBT (nitroblue tetrazolium) used with alkaline phosphatase as substrate tetrazolium, tetranitroblue tetrazolium used with glucose oxidase as substrate, and immunogold with silver enhancement (Leong, 1993 Leong et al, 1997a). 

Goode, D. and Maugel, T. K. (1987) Backscattered electron imaging of immunogold labeled and silver-enhanced microtubules in cultured mammalian cells. J. Electron Microsc. Tech. 5, 263-273. 

Fig. 5A-C. CBi expression on GABAergic terminals in rat somatosensory cortex. CBi receptors [arrowheads] were detected with an antibody directed againsttheC terminusof rat CBi using pre-embedding immunogold with silver enhancement. The boutons are forming symmetric synapses [arrows), characteristic of cortical GABAergic axon terminals. CBi -positive terminals form synapses with pyramidal cell bodies (A), main apical dendrites (B), and fine-caliber dendrite branches [C).Scalebar = 0.5 pm. (Original photomicrograph provided byTamas Freund and Agnes Bodor)...

Burry RW (1995) Pre-embedding immunocytochemistry with silver-enhanced small gold particles. In Hayat MA (ed.) Immunogold-sUver staining principles, methods and applications. Boca Raton, FL CRC Press, pp 217-230. 

Burry. R. W. (1995). Pre-embedding immunsilver-enhanced small gold particles. In "Immunogold-Silver Staining Principles. Methods, and Applications" (M. A. Hayat. cd.), pp. 217-230. CRC Press, Boca Raton. FL. 

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