Silicon tubes

It is very simple to perform batch fermentation in a small flask with a volume of say 200 ml. Now our target is to use a 2 litre B. Braun fermenter. All accessories are shown in Figure 10.5. The fermentation vessel only, as shown in Figure 10.6, with about 250 ml of media without any accessories but with some silicon tubing attached with a filter for ventilation is autoclaved at a 131 °C for 10 minutes at 15psig.9 After that, the system is handled with special care and all accessories attached. Media is separately sterilised and pumped into the vessel. Inoculum is transferred and the batch experiment is started right after the inoculation of seed culture. An initial sample is withdrawn for analysis. 

Glass and silicon tubes with diameters of 79.9-166.3 iim, and 100.25-205.3 am, respectively, were employed by Li et al. (2003) to study the characteristics of friction factors for de-ionized water flow in micro-tubes in the Re range of 350 to 2,300. Figure 3.1 shows that for fully developed water flow in smooth glass and silicon micro-tubes, the Poiseuille number remained approximately 64, which is consistent with the results in macro-tubes. The Reynolds number corresponding to the transition from laminar to turbulent flow was Re = 1,700—2,000. 

Chamberlain LJ, Yannas EV, Arrizabalaga A, Hsu HP, Norregaard TV, and Specter M. Early peripheral nerve healing in collagen and silicone tube imolants Myofibroblasts and the cellular response. Biomaterials, 1998, 19, 1393-1403. 

Another way for fabricating cylindrical DBAs is to use elastomer tubes (Figure 10.12) instead of a dielectric film. Carpi et al. have reported on the studies of cylindrical actuators using silicon tubes with compliant electrodes on the inner and outer walls of the tube. 

Gibson TL, AS Abdul, PD Chalmer (1998) Enhancement of in situ bioremediation of BTEX-contaminated ground water by diffusion from silicone tubing. Ground Water Monit Remed 18 93-104. 

Nonplant cost, 9 527 Nonpoint contamination source, 13 310 Nonpolar adsorbents, 1 674 for gas adsorption, 1 632 Nonpolar solvents, VDC polymer degradation in, 25 717-718 Nonporous dense membranes, 15 799 Nonporous silicone tubing, flow through, 15 722, 723... 

Figure 7 (a) Sartorius absorption model (b) Sartorius dissolution model, a, Plastic syringe b, timer c, safety lock d, cable connector e, silicon tubes f, silicon-O-rings g, metal filter h, polyacryl reaction vessel. 

The concentrated sterilized medium was pumped into the Wavebag with a peristaltic pump through autoclaved silicone tubes. Then distilled water (17.6 L) was pumped in through a disposable, sterile microfiltration capsule. Rocking was started at an angle of 10.5° at 36-37 rpm as well as the temperature regulation, set-point 28 °C. 

A schematic diagram of the automated FIA/DCP/OES system is shown in Fig. 7.6. The carrier liquid is transported using a peristaltic pump, at a rate predetermined to suit the particular analysis. Teflon tubing (internal diameter 0.8 mm) is used where appropriate however, silicone tubing is applied at the roller heads to accommodate both organic and inorganic solvents. Samples are introduced by the autosampler through a loop. The size of the loop is variable for most applications a 600 pi loop is sufficient. 

Figure 9.6 Control of water activity by equilibration via the gas phase or via silicone tubing. 1 gas phase 2 immobilised enzyme 3 reaction medium (substrate in organic solvent) 4 stirring bar, 5 saturated salt solution 6 salt crystals 7 silicone tubing 8 organic solvent (to keep the salt solution solvent saturated).

Silicon tubes and needles (prepumps four nos. and postpumps four nos.) CC LR TC14 TC15... 

Braatz et al.- studied the adsorption of proteins on polyurethane surfaces. They coated silica and silicone tubing with a variety of chemistries. Although they found differences, the polyuretlianes demonstrated minimal protein adsorption given sufficient coating thickness. Correlations between the relative number of hard (isocyanate) and soft (glycol) segments were drawn. More soft segments resulted in less adsorption. 

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