SILAC stable isotope labeling with amino acids in cell

The approach recruited to chemical proteomics in Reference [17] is called SILAC (stable isotope labeling with amino acids in cell culture) and is important in comparative proteomics (Figure 1). SILAC works well with cultured mammalian cells, but prokaryotes defeat it by metabolizing the label (usually supplied in lysine and arginine) into other amino acids. For applications beyond cultured eukaryotic cells, the reductive methylation route to differential labeling [18] is among the alternatives [15]-... 

SILAC Stable-isotope labeling with amino acids in cell culture... 

SILAC stable isotope labelling with amino acids in cell cultmes... 

Guerrero et al. (2006) used this technique along with the quantitative mass spec strategy called SILAC (stable isotope labeling of amino acids in cell culture Ong et al., 2002) to identify the yeast proteins that interact with the 26 S proteasome. 

Everley PA, Krijgsveld J, Zetter BR, Gygi SP (2004) Quantitative cancer proteomics stable isotope labeling with amino acids in cell culture (SILAC) as a tool for prostate cancer research. Mol Cell Proteomics 3(7) 729-735... 

Certain methodologies used in conjunction to protein profiling by mass spectrometry. There are several methods to label proteins that assist their profiling by mass spectrometry. These methods involve labeling of proteins in vitro or in vivo with an isotope. Some of these techniques include Isotope Coded Affinity Tag (ICAT) and Stable Isotope Labeling with amino acids in Cell culture (SILAC). These are described below. 

The sample proteins are usually prepared by a several methods. Among these methods the following are commonly used phosphoprotein isotope-coded affinity tag (PhlAT), isotope-coded affinity tag, (ICAT) and stable isotope labeling with amino acids in cell culture (SILAC). PhlAT introduces isotopes directly into phosphoserine and phosphothreonine residues of the protein. 

Later, Ong et al. introduced a method termed SILAC (stable isotope labeling by amino acids in cell). In SILAC, two cell-culture populations are grown under identical conditions, except that one is supplied with the labeled amino acids (e.g. arginine with six atoms) and the other is with the non-labeled. After five or six doublings, two kinds of amino acids have fully incorporated into proteins. Every peptide pair is separated by the mass shift by the labeled amino acid. This approach cannot be applied to tissues or body fluids, and is... 

Figure 4.10. Proteomic analysis by SILAC. Proteomic analysis by SILAC or stable isotope labeling of amino acids in cell culture utilize de novo metabolic incorporation of stable-isotope-labeled amino acids during protein synthesis. Cells can be cultured with various combinations of stable-isotope-labeled amino acids such as lysine or arginine. Tyrosine has been used in phosphoprotein studies of tyrosine residues. About five or six cell divisions are needed for complete labeling of proteins in cell cultures prior to experimentation. Labeled cells from control and treatment(s) lysates are combined and digested. Quantitation and identification are performed by LC-MS/MS.

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