Shodex OHpak

IV. Shodex OHpak SB-800HQ SERIES A. Column Characteristics... 

The packed columns of Shodex OHpak SB-800HQ series are packed with polyhydroxymethacrylate gels and are designed for use with high-resolution, high-speed aqueous size exclusion chromatography. The packed columns are best suited for the analysis of water-soluble polymers and proteins (Table 6.8). 

The Shodex OHpak SB-800 HQ series is usually not the best choice for the analysis of proteins. However, in some cases, such as when an alkaline... 

TABLE 6.9 Usable Organic Solvent for Shodex OHpak SB-800 HQ Series... 

FIGURE 6.26 Dextran. Column Shodex OHpak SB-805 HQ X 2, 8 mm i.d. X 300 mm X 2. Eluent 100 mM NaNOj. Flow rate 1.0 mL/min. Detector Shodex Rl. Column temp. 30°C. Sample Dextran (Industrial grade). 

FIGURE 6.39 Hydroxyethyl cellulose. Column Left Shodex OHpak SB-806 HQ, 8.0 mm i.d. x 300 mm. Right Shodex Asahipak GF-710 HQ, 7.6 mm i.d. x 300 mm. Eluent 100 mA1 NaNOj. Flow rate 1.0 mL/min. Detector Multi-angle laser light scattering detector. Column temp. 40°C. Sample 0.05%, 200 /iL, Hydroxyethyl cellulose. 

SW series and Alltech Macrosphere series), hydroxylated methacrylate (e.g., TosoHaas TSK PW series. Waters Ultrahydrostyragel, Shodex OHPak), functionalized styrene/DVB (Polymer Laboratories PL aquagel-OH), poly(vinyl alcohol) (Shodex OHPak), and others. 

Molecular weight distributions were examined by high performance size-exclusion chromatography (HPSEC) as described [22] on two serial Shodex OHpak KB-803 and KB-805 columns (0.8 x 30 cm Showa Denko, Japan) with a OHpak KB-800P guard column (0.6 x 5 cm), equilibrated at 1 mL/min in 0.1 M UNO3. The system was calibrated with a pullulan calibration kit (Showa Denko). 

Fig. 2 HPSEC profiles of three RG-II fractions purified from red wine (a), apple juice (b) and tomato juice (c) on Shodex OHpak KB columns. Elution times and Mw (kDa) of the pullulan standards are shown.

Molecular weight distribution was analysed by gel chromatography (0.5 % AG) equipped with a Shodex Ohpak B 805 column (500 8 mm) with phosphate buffer (pH 6.5) using diffe-rential-refractometer/viscometer detection (Knauer). For calibration a pectin series from vibration milling was applied. 

Suppliers of polymeric packings for aqueous size-exclusion chromatography are Polymer Labs (Plgel, UK), Showa Etenko (Shodex OHpak, Japan), Tosoh (TSKgel PW, Japan), and Waters (Ultrahydrogel, USA). 

The Micropak TSK Gel PW, TSK Gel PWXL and Shodex OHpak Q-800, B-800, and KB-800 series are more recently available columns developed for analyzing the acrylamide polymers and other water-soluble polymers in aqueous SEC. The TSK columns have been evaluated by Barth (25), Alfredson et al. (42), Sasaki et al. (43), and Lin and Getman (44). Showa Denko K. K. (45) reported the SEC analysis of PAM by Shodex OHpak columns. The narrow MWD polyacrylamide standards ( =... 

PAM, HYP AM and AM/AA copolymers can be analyzed by TSK Gel PWXL (44), TSK Gel PW (25, 44), Shodex OHpak (25, 45), CPG (31, 32, 33, 34, 38, 41, 46), Sephacryl SIOOO (39), polyvinylpyrrolidone-coated silica columns (36) with an appropriate mobile phase. For cationic acrylamide copolymers, the Gel TSK PW columns (44) have a better separation capability than the Gel PWXL columns. This is probably due to the higher number of residual anionic sites found in PWXL columns (44). When a cationic polyacrylamide is analyzed. 

PAM Polyacrylamide CR 7.9kD-197kD US 7.9kD-725kD Inj. in water with Na2S04 Deionized water with 0.02M Na2S04 / methanol 42 / 58 vol. Polyhydroxy-methacrylate sorbent Shodex OHpak 300x0.8 cm Hybrid LC LCA-LCS PAM with M >20kD are insoluble in the eluent and have limited recovery from the column. Bartkowiak A, Hunkeler D, Berek D, Spychaj T [157]... 

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