Settlement assay barnacle

Rittschof, D., Clare, A.S., Gerhart, D.J., Avelin Mary, S., and Bonaventura, J., Barnacle in vitro assays for biologically active substances toxicity and settlement assays using mass cultured Balanus amphitrite amphitrite Darwin, Biofouliag, 6, 115, 1992. 

For the primary antifouling screening, a barnacle assay is used in most cases. For this test, mass reared cyprid larvae of barnacle species like for example Balanus amphitrite are injected in dishes containing solutions of test compounds in seawater. After one hour, the settlement inhibition in regard to biodde-free controls is determined. 

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