Serology VOLUME

The serological pipet is calibrated through the tip and may be used by adjusting the meniscus to the line that would deliver the desired volume by letting it drain completely (and blowing it out). The Mohr pipet is calibrated only to the lowest calibration line and cannot be drained below this line. 

Fractional volumes of liquid are transferred with graduated pipets, which are available in two types—Mohr and serological. Mohr pipets (Figure 1.4G) are available in long- or short-tip styles. Long-tip pipets are especially attractive for transfer to and from vessels with small openings. Virtually all Mohr pipets are TD and are available in many sizes (0.1 to 10.0 mL). The marked subdivisions are usually 0.01 or 0.1 mL, and the markings end a few centimeters from the tip. Selection of the proper size of pipet is especially important. For instance, do not try to transfer 0.2 mL with a 5 or 10 mL pipet. Use the smallest pipet that is practical. 

There are three types of volumetric pipettes volumetric transfer, measuring, and serological. The differences are based on whether the volume within the pipette is subdivided and if the volume in the tip is included in the calibration (see Fig. 2.19). 

Measuring pipettes come in both Class A and Class B and are available in two styles Style 1 is a standard taper tip, and Style 2 is a long taper tip (Class B only). Serological pipettes are only made to Class B tolerances and have no special styles. Volumes, tolerances, and other data of measuring pipettes are provided in Table 2.17. Information for serological pipettes is provided in Table 2.18. All calibrations were made at 20°C. 

Sephadex G-50 column. Add 20 ml of phosphate buffer to 1 g of Se-phadex G-50 Fine. Let stand at least 3 hr at room temperature, then pour the slurry into a 10-ml disposable glass serological pipette containing a small amount of glass wool at the tip. The bed volume should be about 10 ml. 

A transfer pipette is designed to transfer a known volume of liquid. Measuring and serological pipettes are scored in units such that any volume up to a maximum capacity is delivered. Table 1-12 shows examples of tolerances of various types of these pipettes. 

Early presentation of malabsorption will, however, be more subtle than this list would indicate. There maybe only a slight alteration in volume or consistency of the stool and only mild symptoms attributable to the GI tract. The patient may complain only of anorexia, fatigue, and lack of interest in daily activities. It is in these cases that the physician who suspects malabsorption on clinical grounds will rely on the laboratory to assist in the diagnosis. The initial laboratory investigations are routine tests, abnormalities of which may point to the possibility of malabsorption (e.g., blood hemoglobin concentration mean red cell volume serum concentrations of folate, ferritin, calcium, albumin, and alkafine phosphatase and tests for antibodies in celiac disease I celiac serology]). 

Serological TD Delivery of variable volume 0.1-10 Blow out last dropt... 

The quality of apparatus is crucial. The quality of the pipets directly affects the reliability of the analytical information. It is necessary to use measuring pipets of high quality for reliable measures of volume, e.g., clinical, serological pipets, micropipets, syringe pipets. Syringe pipets must be used for measurement of microliter volumes. In volumetric analysis more reliable burets are necessary. For small quantities of sample, micro- and ultramicro-micrometer burets are recommended. Only well-calibrated pipets and burets will assure the accuracy of volume determination. 

This prospective phase II trial included 29 consecutive patients with 34 unresectable intrahepatic HCC manifestations. The study population comprised 20 men and 9 women the medium age was 66 years (43-79 years) 23 patients had liver cirrhosis grade Child A, and six Child B. Pre-interventional ascites was present in 7 patients (24%). The mean tumor diameter was 4.5 cm (1.5-10.5 cm). The median minimal dose inside the tumor volume was 20 Gy (12-25 Gy) applied as a single fraction. We used 1-13 brachyther-apy applicators per tumor. During follow-up, MRI was performed 3 days, 6 weeks and every 3 months post treatment, supplemented by clinical examinations and serological liver function tests. Primary endpoints were local tumor control after 12 months as well as safety. Secondary endpoints were progression-free survival and overall survival. 

However, the volume of information inscribed in 1200 nucleotides is insufficient for spontaneous propagation of the virus in plant cells, and it can reproduce only as the satellite of another virus—virus of tobacco necrosis, possessing RNA with a molecular wei t of 2 X 10. Proteins of these viruses are serologically different, but the basic virus evidently enables certain factors to be synthesized which are equally essential for the formation of both viruses. RNA synthesis in the host cells takes place on DNA templates and is catalyzed by DNA-dependent RNA-polymerase. It may be assumed that a special factor—the enzyme RNA-replicase— which is not used for reproduction of the satellite virus is essential for synthesis of virus RNA on RNA templates (autoreplication of RNA). 

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