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Affinity chromatography lectins

Lectin affinity chromatography may be used to purify a range of glycoproteins. Lectins are a group of proteins synthesized by plants, vertebrates and a number of invertebrate species. Especially high levels of lectins are produced by a variety of plant seeds. Plant lectins are often termed [Pg.150]

Lectin Source Sugar specificity Eluting sugar [Pg.151]

Con A Jack bean seeds a-D-Mannose, a-D-glucose a-D-Methyl mannose [Pg.151]

WGA Wheat germ A-Acetyl-P-D-glucosamine A-Acetyl-P-D-glucosamine [Pg.151]

Although lectin affinity chromatography may be utilized to purify a variety of glycoproteins, it has not been widely employed for a number of reasons ... [Pg.151]

Protein A chromatography Lectin affinity chromatography Immunoaffinity chromatography... [Pg.142]

Lectin affinity chromatography may be used to purify glycoproteins Immobilized antibodies may be used as affinity absorbants for the antigen that stimulated their production (e.g. purification of factor VIII using immobilized anti-factor VIII antibodies)... [Pg.142]

Serial lectin affinity chromatography Systemic Inpns erythematoses Yeast osmosensor... [Pg.22]

R. Qiu, F.E. Regnier, Use of multidimensional lectin affinity chromatography in differentialglycoproteomics. Anal. Chem., 77 (2005) 2802. [Pg.543]

Baylin SB, Wieman KC, O Neil JA, Roos BA. Multiple forms of human tumor calcitonin demonstrated by denaturing polyacrylamide gel electrophoresis and lectin affinity chromatography. J Clin Endocrinol Metab 1981 53 489-97. [Pg.1944]

Gonchoroff DG, Branum EL, O Brien JF. Alkaline phosphatase isoenzymes of liver and bone are incompletely resolved by wheat-germ-lectin affinity chromatography. Clin Chem 1989 35 29-32. [Pg.1951]

Lectin affinity chromatography Used in techniques such as immobilized lectin affinity chromatography [268]... [Pg.192]

Purify ACE by fractionation on DEAE-cellulose, Ca phosphate gel chromatography, elution from Sephadex G-200 and lectin affinity chromatography. The MW varied with glycosidation and is higher by gel filtration. It contains one atom of Zn/mol and has Km values for hydrolysis of hippurylhistidinylleucine and angiotensin I of 2.3 and 0.07 mM, and turnover of 15,430 and 792 mol/min/mol at 37°, respectively. The activity is inhibited by EDTA and increased amounts of Ca ions but required Ca ions. [Das Soffer J Biol Chem 250 6762 1975, Reviewed by Ehlers Riordan Biochemistry 28 5311 19891]... [Pg.609]

See other pages where Affinity chromatography lectins is mentioned: [Pg.192]    [Pg.196]    [Pg.214]    [Pg.259]    [Pg.270]    [Pg.150]    [Pg.214]    [Pg.516]    [Pg.237]    [Pg.198]    [Pg.62]    [Pg.89]    [Pg.268]    [Pg.281]    [Pg.447]    [Pg.147]    [Pg.439]    [Pg.114]    [Pg.213]    [Pg.228]    [Pg.234]    [Pg.105]    [Pg.208]    [Pg.133]    [Pg.742]    [Pg.1748]    [Pg.104]    [Pg.115]    [Pg.44]    [Pg.94]    [Pg.190]    [Pg.484]    [Pg.261]    [Pg.46]    [Pg.210]    [Pg.427]   
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See also in sourсe #XX -- [ Pg.210 ]

See also in sourсe #XX -- [ Pg.269 ]

See also in sourсe #XX -- [ Pg.86 ]



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