Sequential injection analysis principles

Among the variations of the SIA system, sequential injection chromatography (SIC) is the most recent technique and it has been proposed by Huclova et at. [46]. These authors describe the use of a porous monolithic column of silica as the stationary phase coupled to SIA for the separation and quantification of salicylic acid and methyl salicylate in pharmaceutical products. 

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A. Economou, RD. Tzanavaras, D.G. Themelis, Sequential-injection analysis Principles, instrument construction, and demonstration by a simple experiment, J. Chem. Edu. 82 (2005) 1820. 

To alleviate these drawbacks, alternative methodologies relying on the continuous provision of fresh extractant volumes to the solid sample under mvestigation have been developed, characterized, and contrasted with the classical end-over-end extraction procedures. The fundamental principles of these novel, dynamic (nonequilibrium) strategies, based primarily on the use of continuous-flow analysis (Ruzicka and Hansen, 1988), flow injection analysis (Ruzicka and Hansen, 1988 Trojanowicz, 2000 Miro and Frenzel, 2004b), or sequential injection analysis (Ruzicka and Marshall, 1990 Lenehan et al., 2002), are described in detail below, and their advantageous features and limitations for fractionation explorations are discussed critically. 

Describe the principles of sequential. injection analysis and how it differs from conventional flow injection analysis. 

See FLOW ANALYSIS Overview. FLOW INJECTION ANALYSIS Principles Instrumentation Detection Techniques Environmental and Agricultural Applications Clinical and Pharmaceutical Applications Industrial Applications. SEGMENTED FLOW ANALYSIS. SEQUENTIAL INJECTION ANALYSIS... 

Ruzicka J, Gubeli T (1991) Principles of stopped-flow sequential injection-analysis and its appbcatitm to the kinetic determmatirai of traces of a proteolytic-enzyme. Anal Chem 63 1680-1685... 

This approach uses a kinetic sequential principle to carry out multicomponent CL-based determinations. In fact, when the half-lives of the CL reactions involved in the determination of the analytes in mixture are appreciably different, the CL intensity-versus-time curve exhibits two peaks that are separate in time (in the case of a binary mixture) this allows both analytes to be directly determined from their corresponding calibration plots. In general, commercially available chemiluminometers have been used in these determinations, so the CL reaction was initially started by addition of one or two reaction ingredients. Thus, in the analysis of binary mixtures of cysteine and gluthatione, appropriate time-resolved response curves were obtained provided that equal volumes of peroxidase and luminol were mixed and saturated with oxygen and that copper(H) and aminothiol solutions were simultaneously injected [62, 63],... 

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