Separator Shodex

The results in Table 17.8 indicate that for polyethylene oxide standards Shodex columns have better separation efficiency than the TSK GM-PW and... 

The difference in retention times between the 920,000 PEO and the 21,000 PEO in Table 17.9 can be used as a measure of the void or pore volume that effectively provides the linear separation range for these columns in water and in a water/methanol mixture. The better separation efficiency of the Shodex columns over the TSK columns is partially related to the larger void volumes of the Shodex columns than the TSK columns. The difference in void volumes for the Shodex, TSK GM-PW, and TSK GM-PWxl columns is partially attributed to the difference in the inner diameters of the three columns, which are 8 (Shodex), 7.8 (TSK GM-PW) and 7.5 (TSK GM-PWxi.) mm. Table 17.9 also... 

Figure 4.7 Anion exchange separation of carboxylic acids in red wine. Column, Shodex C811, 100 cm x 7.6 mm i.d. eluent, 3 mM perchloric acid flow rate, 0.9 ml min-1 temperature, 60 °C detection, reaction detection using chloro-phenol red at 430 nm. Peaks 1, citric acid 2, tartaric acid 3, malic acid 4, succinic acid 5, lactic acid 6, formic acid and 1, acetic acid.

A HPLC system that separates various preservatives was described by Shodex [35]. The system consists of a Shodex Rspack DS-613 column, and a Shodex UV detector. A mobile phase mixture of 0.05 M K2HPO4 /... 

The organic acids were analyzed with an HPLC system consisting of degassing unit (Gastorr GT-103),pump (Pharmacia LKB), autosampler (Pharmacia, LKB), precolumn (Shodex R Spak KC-LG), separation column (Shodex R Spak KC-811), water bath (Julabo U3) and a UV detector (Soma Optics LTD S-3702). The data monitoring was performed using a PC-chromatography data system Andromeda 1.6 (Techlab). 

Figure 6-10. Example of a separation of dietary fiber mix on GPC column from Shodex. (Reprinted from reference 4, with permission from Phenomenex.)...

SEC System. A Spectra Physics IsoChrom pump controlled the THE flow in the SEC part of the 2D instrument. Two SDV 5-jLtm SEC columns with 1000 and 10 A porosity (PSS) were used for size separation of the HPLC fractions. UV (SP 8450, Spectro Physics) and RI (Shodex SE 61, Diisseldorf, Germany) detection allowed lor conventional or multiple-detection data processing of detector traces. Eor the polyester analysis the SEC columns (50 and 100 A) were operated in acetone as eluent. 

Traces of iodide can be separated as Agl by co-precipitation with silver chloride [3-5]. Trace amounts of iodide present in brines were retained on the anion-exchangers Amberlite IRA 458 [6] or Shodex 1-524 A [7]. 

Hot water extraction (TCA treatment for blood plasma), AE separation (polymer-based Shodex Asahipak ES-502N 7C column) with non-buffering mobile phase (15 mmol L citric acid adjusted to pH 2 with HNO3) ICP-MS detection... 

PAM, HYP AM and AM/AA copolymers can be analyzed by TSK Gel PWXL (44), TSK Gel PW (25, 44), Shodex OHpak (25, 45), CPG (31, 32, 33, 34, 38, 41, 46), Sephacryl SIOOO (39), polyvinylpyrrolidone-coated silica columns (36) with an appropriate mobile phase. For cationic acrylamide copolymers, the Gel TSK PW columns (44) have a better separation capability than the Gel PWXL columns. This is probably due to the higher number of residual anionic sites found in PWXL columns (44). When a cationic polyacrylamide is analyzed. 

Overlays of SEC chromatograms of five commercial grades of PVP using the Shodex KB-80M linear column with a mobile phase of 20 80 (vol/vol) MeOH/HjO with 0.1 M LiNOj and the Ultrahydrogel linear column with a mobile phase of either 20 80 (vol/vol) MeOH/HjO with 0.1 M LiNOj or 50 50 (vol/vol) MeOH/HjO with 0.1 M LiNOj are shown in Figures 5, 6, and 7. Adequate separation of all commercial grades of PVP can be obtained from all three systems. 

Figure 2.11 Separation of alkylphenones on a CIS-modified polyvinyl alcohol (PVA) phase over a rwide range of temperature. Column Shodex ET-RP1 4D 5 pm, 250 mm X 3 mm eluent H20/Me0H/25/75...

SEC experiments were carried out on a Watters 150CV instrument (Waters Associates, Milford, Massachusetts, U.S.A.) equipped with both differential refractive index single-capUlary viscometer detectors. The solvent/mobile phase was H20/0.02% NaNs, at the flow rate of 1.0 ml/ min. Pump, solvent, and detector compartments were maintained at 50°C. Separation occurred over a column bank consisting of three analytical columns preceded by a guard column Shodex KB-G, KS-802, KS-803, and KB-804 (Phenomenex, Torrance, California, U.S.A.). Universal calibration was performed using a series of oligosaccharides (Sigma, St. Louis, Missouri, U.S.A.), and PuUuIan Standards (American Polymer Standards, Mentor, Ohio, U.S.A., and Polymer Laboratories, Amherst, Massachusetts, U.S.A.). 

Figure 4.35 Separation of common inorganic cations on Shodex 1C YS-50. Column dimensions 125 mm X4.6 mm i.d. column temperature 40°C eluent 4 mmol/L methanesulfonic acid flow rate 1 mlVmin detection ...

Figure 8.83 Separation of oligosaccharides using gradient HILIC and charged aerosol detection. Column Shodex Asaipak NH2P-5O 4E, 5 (im column dimensions 250 mm x 4.6 mm i.d. column temperature 35 °C eluent (A) MeCN and (B) water gradient 70% A to...

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