Separator Prontosil

FIGURE 2.4 Separation of strong bases on a classical RP and on a shielded phase. Columns Prontosil CIS H Prontosil CIS ace EPS (150x4.0mm). Mobile phase MeOH/phosphate buffer 20mM pH=7, 65/35 v/v, 40°C. Analytes 1, uracil 2, protriptyline 3, nortriptyline 4, doxepine 5, imipramine 6, amitriptyline 7, trimipramine S, clomipramine. 

FIGURE 2.10 Demonstration of shape influence on solute retention. Separation of fatty acid esters of identical chain length hut different position of double bonds. Column Prontosil SH 150x4mm mobile phase acetonitrile-water 9-1, v-v. Samples co3-docosa hexaenic acid ethylester (DHAEE) o)3-docosa pentaenic acid ethylester (DPAEE) o)6-docosa pentaenic acid ethylester. 

FIGURE 2.17 Separation of strong bases (anti-depressive) on a classical RP and shield phases. Influence of organic modulator. Columns Prontosil 120 C8 SH and Prontosil C8 ace-EPS (shield phase). Conditions and samples are summarized in Table 2.2. 

Figure 5.2.3 depicts the HPLC chromatogram of a tomato peel extract monitored by UV absorbance at 469 nm. The separation was performed on a 150 x 4.6 mm C30 column (ProntoSil, 3 xm, 200 A, Bischoff, Germany) at room temperature and a flow rate of 1 ml/min with a binary mixture of acetone/ water, developed for LC-NMR experiments. The 50-min gradient elution was performed in four steps, i.e. (1) an initial 3 min with 75/25 (v/v) acetone/water, (2) a 24-min gradient to 100% acetone, (3) an isocratic step from 27 45 min with 100% acetone, and (4) a 2-min gradient back to the initial conditions. 

The Prontosil column is very similar to, although not identical with, Genesis. It could be used as an alternative if the latter one is not available. On the other hand, the Platinum is very different and cannot be used as a substitute. However, it could be interesting to use it in order to check if all peaks of a mixture are separated in the course of selectivity tests during validation. 

Figure 10.8 Separation of hydrophilic compounds on three different octadecyl columns [redrawn with permission from M.R. Euerby and P. Petersson, J. Chromatogr. A, 994, 13 (2003)]. Stationary phases Prontosil C18 AQ (similar, Fs = 7.7), Genesis AQ (reference) and Platinum EPS C18 (different, Ag —58). Conditions columns, 15cm x 4.6mm i.d. mobile phase, 1 mlmin potassium dihydrogen phosphate pH 2.7 in water-methanol (96.7 3.3) temperature, 6O C UV detector, 210nm. Peaks 1—nicotine 2 = benzylamine 3 = procainamide 4 = terbutaline 5 —salbutamol 6 —phenol. 

Prontosil AQ, which is more hydrophilic than Prontosil Cjg, is better at separating the polar pair phthahe acid/terephthalic add. Otherwise, these two phases are quite similar. 

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