Separation using HPLC

Plasma, Urine ( -aminolevuli nic acid) Derivatization of -aminolevulinic acid with formaldehyde and acetylacetone to form fluorescent compounds separation using HPLC HPLC/FL 3 pg/L No data Oishi et al. 1996... 

A major advance in this area came in 1992 when Harger and co-workers (1992) reported the direct mutagenicity of vapor- and particle-phase organics separated using HPLC in samples collected during the... 

The mixture of hydrazones formed from the reactions of the set of carbonyl compounds commonly found in air can then be separated using HPLC and detected using light absorption at 254 or 365 nm (e.g., see Kuntz et al., 1980 Lipari and Swarin, 1982 and Kuwata et al., 1983). 

The analysis of the natural carotenoid pigments found in orange juice, for example, is extremely complex and should be left to an expert laboratory. However, the quantitative analysis of added /3-carotene and/or /3-apo-8 -carotenal to an orange drink or dilutable is much easier as pure standards are commercially available and these compounds can be readily separated using HPLC. 

Morris JE, Tolppi GC, Dunlap CJ, Carr PW, Flickinger MW (1994) Protein separation using HPLC and fluidized bed zirconia supports. Proceedings of the ACS Meeting, San Diego... 

The enantiomers of pantoprazole were separated using HPLC at 40°C on Chiralcel OJ-R columns with 25% acetonitrile and 50 mM NaC104 (3 1 v/v) as the mobile phase [11]. Elution was performed at a flow rate of 0.5 mL/min, and detection was at 286 nm. Lichroprep PR-2 was used as a pre-column, and Lichrospher 100 RP-18 was used as a guard column. 

In another approach using stable isotopes to measure retinoids in human serum, Parker and coworkers (296,297) studied the absorption and metabolism of C-labeled P-carotene using GC-isotope ratio mass spectrometry. Retinol and retinyl esters were separated using HPLC, the retinyl esters were hydrolyzed to retinol, and each retinol sample was analyzed using GC-isotope ratio mass spectrometry. As the retinol peak eluted from the GC, it was combusted to carbon dioxide, and the C02/ C02 ratio was measured using an isotope ratio mass spectrometer. In a similar way, lutein isolated from C3 and C4 plant sources was hydrogenated to perhydrocarotene which was subjected to GC-combustion-inter-faced isotope ratio-mass spectrometry (GC-C-IR-MS) to determine natural abundance C in lutein (298). 

Fluoroquinolone Antibiotics. Ionic liquids differing in the length of the alkyl chain were tested as mobile phase additives for the separation using HPLC of fluoroquinolone antibiotics (15). The materials are listed in Table 1.4. Fluoroquinolone antibiotics are shown in Figure 1.9... 

Cooper et al. described the UHPLC-MS method, which allowed the separation of six procyanidins within 3 min, while the same mixture of compounds was separated using HPLC within 20 min (17). In yet another work, the comparison of HPLC... 

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