Sendai virus particles

To date, we have been developing FL. The FL, prepared by fusing simple liposomes with Sendai virus particles can fuse with the cell membrane like the native virus particle and deliver their contents directly and efficiently into the cytoplasm both in vitro and in vivo as well as showing superior safety (Fig.l). 

The presence of informosomelike RNPs has been demonstrated also in the case of Ehrlich ascites carcinoma cells infected with Sendai virus (Volkova et al., 1969). The cytoplasmic extracts of the cells labeled for 30 minutes with uridine contain virus-specific RNA in the form of particles with a sedimentation coefficient 45S (the sedimentation coefficient of the complete virus equals 57S). The buoyant density of the RNP peak in CsCI equals 1.43 to 1.44 g/cm. Although these properties are compatible with the idea that they are informosome, the particles, and in particular their protein component, should be characterized in more detail before reaching a definite conclusion. Recently SOS virus RNA-containing particles with p = 1.40 g/cm have been found in HeLa cells infected with poliovirus (Huang and Baltimore, 1970), although the authors have some doubts about the reality of these complexes. 

Volkova, M. Y., V. M. Zaides, and V. G. Zaslavsky. 1969. Slowly sedimenting particles present in cytoplasmic extract of Ehrlich ascites cells infected by Sendai virus. Molec. Biol. (U.S.S.R.), 3 635-638. 

Figure 1. Calibration curves for three Polyol columns. Calibration curves of three SE-HPLC columns (100 mm X 4.6 mm i.d.) (Serva, Heidelberg, FRG). Particle size and pore size respectively were (a) 5 jim, 50 nm (b) 5 >tm, 30 nm (c) 10 /rni, 30 nm. The flow-rate was 0.1 ml/min and 10 /rl samples of the tetramer of Sendai virus protein HN (272 kd), BSA (68 kd), ovalbumin (43 kd) and trypsin-inhibitor (20 kd) were applied to the column.

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