Semi-preparative high performance liquid chromatography

Shin, T.S. and Godber, J.S. 1994. Isolation of four tocopherols and four tocotrienols from a variety of natural sources by semi-preparative high performance liquid chromatography. J. Chromatogr. A 678 49-58. 

Netting, A.G. Duffleld, A.M. Pentafluorobenzyl esters as derivatives for the semi-preparative high-performance liquid chromatography of fatty acids, J.Chromatogr., 1983,257,174-179. [derivatization SPE normal phase stearic acid oleic acid linoleic acid linolenic acid]... 

Hennion, M.C., J.C. Thieblement, R. Rosset, P. Scribe, J.C. Marty, and A. Saliot. 1983. Rapid semi-preparative class separation of organic compounds from marine lipid extracts by high-performance liquid chromatography and subsequent quantitative analysis by gas chromatography. J. Chromatogr. 280 351-362. 

R.R. West and J.H. Cardellina, Semi-preparative Separation of Polyhydroxyl-ated Sterols Using a P-cyclodextrin High-Performance Liquid Chromatography Column, J. Chromatogr., 539(1991)15. 

Sample preparation Condition a 3 mL 200 mg Bond Elut CIS SPE cartridge with two 3 mL portions of MeOH and two 3 mL portions of 5% acetic acid. Mix 500 p,L serum with 500 p.L 10% acetic acid, add to the SPE cartridge, wash with 3 mL water, elute with four 500 p,L portions of MeOHrwater 60 40. Evaporate the combined eluates to dr3mess under a stream of nitrogen, reconstitute the residue with 250 iL water, filter (0.22 p,m). Inject a 100 p,L aliquot onto column A and elute to waste with mobile phase A. After an unspecified time, backfiush the contents of column A onto column B using mobile phase B. Monitor the effluent from column B. (Sato,K. Kobayashi,K. Moore, C.M. Mizuno,Y. Katsumata,Y. Semi-quantitative analysis of cefaclor in human serum by capillary high performance liquid chromatography/fast atom bombardment mass spectrometry. Forensic Sci.Int. 1993, 59, 71-77.)... 

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