SeMet

Semet-Solvay The Semet-Solvay Engineering Corporation, Syracuse, NY, was best known for its coke-oven technology, developed from the end of the 19th century. The eponymous process was a cyclic, non-catalytic process for making fuel gas from oil. 

Albarede F, Luais B, Fitton G, Semet M, Kaminski E, Upton BGJ, Bachelery P, Cheminee JL (1997a) The geochemical regimes of Piton de la Foumaise volcano (R union) during the last 530.000 years. J Petrol 38 171-201... 

If a methionine auxotroph is not used, the methionine within the culture can be replaced by SeMet using a methionine biosynthesis inhibition or poisoning method such as that outlined in Protocol 2.7. This method has the advantage of using any E. coli strain available and thus the initial growth of the culture is not reduced, however the method is more laborious than using the methionine auxotroph. 

A single colony Is Inoculated Into 100 ml of SeMet media containing antibiotics (Molecular Dimensions). The SeMet media consists of 100 ml SeMet Base, 5 ml nutrient mix, and 0.4 ml 10 mg/ml selenomethionine In a 250 ml shake-flask. The culture Is Incubated at 37°C and 225 rpm overnight ( 16 h). 

The supernatant Is removed and the pellet Is Inoculated Into 1L of SeMet media (1L SeMet Base, 50 ml nutrient mix, and 4 ml 10 mg/ml selenomethionine) which contains antibiotics. 

A single colony is inoculated into 100 ml of glucose-free SeMet media (Molecular Dimensions) containing antibiotics and incubated at37 C and 225 rpm overnight ( 16h). 

SeMet labelled protein using the auxotroph, however on auxotroph B834, however at Stage 4, 9 ml 10 mg/ml... 

Giletti B.J., Semet M.P., and Yund R.A. (1978) Studies in diffusion. III oxygen in feldspars an ion microprobe determination. Geochim. Cosmochim. Acta 42, 45-57. 

Jambon A. and Semet M.P. (1978) Lithium diffusion in silicate glasses of albite, orthoclase, and obsidian compositions an ion-microprobe determination. Earth Planet. Sci. Lett. 37, 445-450. 

Shimizu, N., Semet, M. P. and Allegre, C. J. (1978) Geochemical applications of quantitative ion microprobe analysis. Geochimica et Cosmochimica Acta, 42, 1321-1334. 

Figure 9.20 Different selenium species determined by HPLC-ICP-MS in leaves, stems and roots of dill (1) Se (VI) (2) Se (IV) (3) SeCys2 (4) MeSeMet (5) MeSeCys (6) SeMet. (O. Cankur, S.K.V. Yathavakilla, J.A. Caruso, Talanta, 70, 784 (2006). Reproduced by permission of Elsevier.)...

Fig. 4. Chromatogram obtained for separation of SeCys, SeMet, and TMSe+ (100 xgl 1 as Se) under optimum experimental conditions. Reprinted from Munoz Olivas et al. [54] by permission of The Royal Society of Chemistry.

SeCys, SeMet, TMSe Aqueous matrix The storage in the dark at pH 4.5 in Pyrex containers at both 4°C and 20°C The storage in the dark at 4°C in PP containers 1 year 6 weeks 45 46... 

A commercially available semetic phase composed of biphenylcarbox-ylate ester attached to the polysiloxane backbone of a fused silica column has been shown to be ideal for the separation of geometric isomers of polycyclic aromatics of various classes of compounds [14,15]. This column exhibits a wide semetic temperature range of 100-300°C and has been shown to be stable to at least 280°C. Liquid-crystal stationary phases have also been employed in SFC [16]. 

The X REL1 crystal structure, which consists of residues 52-316 corresponding to the N-terminal domain, was resolved using the SeMet multiple-wavelength anomalous dispersion method (9). The selenomethionines used for this method should be replaced with methionines to obtain the original form of the protein for simulations (see Note 2). 

Three such selenoaminoacids were separated and identified in a study by Olivas et al. [42]. Selenomethionine (SeMet), selenocystine (SeCys), and the trimethylselenomium ion (TMeSe+) were separated by using an anionic counter... 

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