Multiple wavelength Anomalous Dispersion

Several structurally different types of HNLs occur in nature, which likely originated hy convergent evolution from different ancestral proteins. The enzyme from almond (PaHNL) was first crystallized in 1994 and the structure was solved by multiple wavelength anomalous dispersion of a mercury derivative. The first 3D structure analysis of PaHNL was performed in 2001. ° (7 )-PaHNL from almond uses FAD as cofactor and is related to oxidoreductases it exhibits HNL activity only in the oxidized form of FAD." ... 

The X REL1 crystal structure, which consists of residues 52-316 corresponding to the N-terminal domain, was resolved using the SeMet multiple-wavelength anomalous dispersion method (9). The selenomethionines used for this method should be replaced with methionines to obtain the original form of the protein for simulations (see Note 2). 

M. Helliwell, J.R. Helliwell, V. Kaucic, N.Z. Logar, L. Barba, E. Busetto, and A. Lausi, Determination of the Site of Incorporation of Cobalt in CoZnPO-CZP by Multiple-wavelength Anomalous-dispersion Crystallography. Acta Crystallogr., Sect. B Struct. 

MWPCs have been successfully used at LURE and Stanford for multiple wavelength anomalous dispersion phasing measurements (see chapter 9). An MWPC has also been used to record Laue diffraction patterns at VEPP-3 (Gaponov et al 1989a,b,c). 

Multiple wavelength anomalous dispersion based on selenomethionines introduced by genetic engineering [90H1]. PROFFT 0.198 0.015 A 0.034 A 1... 

Energy-dispersive mode for multiple wavelength anomalous... 

The anomalous components of the total scattering are wavelength dependent and the use of radiation close to an absorption edge may increase or optimise the contribution due to the anomalously scattering atoms. Ramaseshan (1962) pointed out that data collected at multiple wavelengths optimising the anomalous dispersion effects would improve the quality of phase determination. 

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