Selection and Screening Methods

Selection and screening methods are effective tools for shidying macromolecular interactions. Valuable methods are the yeast-based one-hybrid and two-hybrid systems (for smdy-ing protein-DNA and protein-protein interactions, respectively) and bacterial-based phage display methods (for studying either type of interaction). These systems have been used to... 

Spudich, J. L., and Spudich, E. N. (1995) Selection and screening methods for halobac-terial rhodopsin mutants. CSH Laboratory Press, Cold Spring Harbor, New York. 

Solvent selection and screening approaches can be divided into two levels of analysis. The first level focuses on identification of functional groups or chemical famihes that are hkely to give favorable solvent-key component molecular interactions. The second level of analysis identifies and compares individual-candidate solvents. The various methods of analysis are described briefly and illustrated with an example of choosing a solvent for the methanol-acetone separation. 

About 4Vn of the population of industrialized countries has diabetes, and rapid, selective, and reliable methods for the determination of blood glucose are of utmost importance for diabetes screening and treatment. Worldwide a potential market for 500 million glucose sensors is anticipated 335. ... 

Once a target for antibacterial intervention has been selected and validated, a path to obtaining inhibitors must be chosen. Assays can be cell based or in vitro, actual or virtual, and screening methods can be high-throughput, focused, or anywhere in between. The size and type of the net to be cast will determine not only the quantity of hits available for characterization but often will determine the quality and type. 

Select and screen MCCs and check their CO release rate, cell toxicity, and anti-inflammatory activity according to the parameters and methods set by the research on CORM-2 and CORM-3. 

Complex lipids (e.g., phospholipids and glycosphingolipids) do not migrate under these conditions, and they can then be detected or quantified only as if they were a single lipid class. Yet, TLC can be used as a rapid screening tool, prior to the application of more selective and sensitive methods. 

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