Selectins distribution

Plasma was separated from heparinized blood samples, ahquoted and stored at — 80°C. All samples were then analyzed simultaneously with identical hatches of antibodies and other reagents. ELISAs for the detection of sICAM-1, sE-selectin and IL-12 were performed with ELI-Pairs (Diaclone, France distributed by Holzel, Germany). 96-well Maxisorp plates (Nunc) were coated overnight with capture antibodies at 4°C. After 1 h sample incubation, detection was performed with biotinylated anti-ICAM-1, anti-sE-selectin or anti IL-12 detection antibodies followed by streptavidin-horse raddish peroxidase and a TMB color reaction. Light absorption was detected with a Spectra ELISA reader at 450 nm. 

Vachino, G, Chang, X-J, Veldman, G M, Kumar, R, Sako, D, Eouser, L A, Bemdt, M C, Cumming, D A, P-selectin glycoprotein ligand-1 is the major counter-receptor for P-selectin on stimulated T cells and is widely distributed in non-functional form on many lymphocytic cells, J. Biol Chem., 270, 21966-21974, 1995. 

Fig. 8. Distribution of selectins and their glycoprotein ligands. Reprinted with permission from Rodger P. McEver, Selectin-carbohydrate interactions during inflammation and metastasis, Glycoconjugate J.. 14 (1997) 585-591 (Ref. 208) (1997). Kluwer Academic Publishers.

Distribution and binding specificity of selectins (for references see the text)... 

Table III-l. Selectin and Integrin Distribution in the Endothelium and Leukocyte...

In a prospective study, fasting serum and anthropometric measures were obtained from 45 patients with first-episode psychosis and 41 healthy adults of similar age, ethnicity, and sex [32 f. At baseline, the distributions of cardiovascular risk markers were similar and the percentages of young patients with first-episode psychosis and healthy controls who were overweight/obese, dyslipidemic, hyperglycemic, and hyperinsulinemic did not differ. At 24 weeks, compared with baseline, 16 of the patients with psychosis who continued to take the same antipsychotic medication had statistically significant increases in BMl, glucose, insulin, cholesterol, leptin, and E-selectin, and a reduction in adiponectin. 

The precise distribution of the two orientations and the presence of other local minima in aqueous solution is still a matter of dispute. Upon binding to E-selectin only one of the two major conformational families generated by different orientations of the a-D-Neu5Ac-(2—>3)-P-D Gal hnkage is recogniz. The bound conformation is characterized by the observation of a trNOE between H3-Gal and H8-Neu5Ac, whereas no interaction is observed between H3-Gal and H3ax-... 

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