Seed storage methods

Severian,P., Devinek,F. (1986). Seed potato (Solanum tuberosum) storage methods and subsequent field production in New Caledonia. Tropical Agriculture, 63, 52-56. 

Freitas RL, Ferreira RB, Teixeira AR (2000). Use of a single method in the extraction of the seed storage globulins from several legume species application to analyse structural comparisons within the major classes of globulins. Int. J. Food. Set Nutr., 51 341-352. 

Although low levels of methylxanthines have been detected in the leaves and flowers of T. cacao, the primary storage location is within the seed or bean.16 The cocoa bean is the major natural source of the methylxanthine theobromine, but contains only small amounts of caffeine. Theophylline has been detected in cacao beans, but at such low concentrations that its presence generally is ignored. Together, theobromine and caffeine account for up to 99% of the alkaloid content of T. cacao beans. Alkaloid content is affected by genetic makeup, maturity of beans at harvest, and fermentation process. Analytical methodology also is partially responsible for some of the disparity in methylxanthine values since many early methods were unable to separate theobromine and caffeine. 

Independent of the method of storage, the revitalization of the microorganisms requires several steps. Starting from the test-tube or agar plate scale, the scale up proceeds stepwise via laboratory inoculums to seed fermenters, which produce enough cells to feed production fermenters. 

Ideally, cottonseed should be stored at a moisture content of less than 10% (49). Dehulled seeds should contain no more than 9% moisture and 1% FFA (109). Prior to storage, cottonseeds must be sampled for moisture analysis. The American Oil Chemists Society (AOCS) method Aa 3-38 specifically describes a procedure for... 

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