Screening methods fluorescence

The ability to display proteins on the surface of an organism has been exploited as a screening method, typically in conjunction with fluorescence-activated cell sorting (FACS) [37,47,48]... 

Separation and detection techniques for antibacterials in food mainly focus on the use of LC coupled to MS or tandem MS. Nevertheless, recent studies have suggested capillary electrophoresis coupled to laser-induced fluorescence (LIE) as a way of improving sensitivity [49], HRLC coupled to microTOF-ESI-MS as a highly selective, sensitive, and quick screening method for 100 veterinary drugs in fish, meat, and egg samples [195], and nanoscale LC coupled to UV or ion trap MS, with LODs in the range 0.01-0.51 pg/L (nanoLC-MS) and the possibility that even lower limits could be achieved by using triple quadrupole MS [59]. 

The incidence of tetracycline residues in turkeys has been also studied recently (13). During meat inspection, suspicious flocks were identified by fluorescence detection in bones. A total of 85 flocks showed significant fluorescence in bones. When bones, liver, kidney, and muscle of these animals were analyzed for tetracyclines, residues of tetracycline, chlortetracycline, oxytetracycline, or doxycycline were found in 83 flocks. These results suggest that the detection of fluorescence in bones might be a useful screening method for tetracycline residues in poultry carcasses. 

The advantage of fluorescence-based assays is their high sensitivity. It is therefore perhaps surprising that few such systems have been developed for evaluating the enantioselectivity of enzyme-catalyzed reactions. Fluorescence as a detection method is used in an enzyme-coupled assay [26] (see Section 9.3.4.3) and in the capillary array electrophoresis [25] (see Section 9.3.6.5). If several substrates need to be screened simultaneously, fluorescence-based substrate arrays as enzyme fingerprinting tools can be used, although enantioselectivity still needs to be addressed [26e],... 

To address the low throughput limitation associated with most screening methods, various fluorescence-activated cell sorting (FACS) based screening methods have been developed. Unlike the above mentioned screening methods, FACS can analyze and sort up to 100,000 cells per second in a quantitative manner... 

In the mid 1980s, Krahn and co-workers (1984) developed a moderately inexpensive, rapid screening method for estimating the relative amount of PAH metabolites present in fish bile. Their method used high-performance liquid chromatography with fluorescence detection (HPLC-F) for estimating bile metabolites. In brief, fluorescent... 

Giessing AMB, Mayer LM, Forbes TL (2003) Synchronous fluorescence spectrometry of 1-hydroxypyrene a rapid screening method for identification of PAH exposure in tissue from marine polychaetes. Marine Environmental Research 56 599-615. 

Fluorescent screens are made of a thin layer of zinc sulfide, containing a trace of nickel, mounted on a cardboard backing. Under the action of x-rays, this compound fluoresces in the visible region, i.e., emits visible light, in this case yellow light. Although most diffracted beams are too weak to be detected by this method, fluorescent screens are widely used in diffraction work to locate the position of the primary beam when adjusting apparatus. 

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