Screening and Microbiological Aspects

Different lyophiHzed micro-organisms, ranging from bacteria (46 strains) to yeasts (42 strains) and molds (15 strains), were screened for the hydrolysis of a number of esters (acetate, butyrate, and caprylate) of geraniol and u-hexanol [4]. 

Modem Biocatalysis Stereoselective and Environmentally Friendly Reactions. Edited by Wolf-Dieter Fessner and Thorleif Anthonsen Copyright 2009 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim ISBN 978-3-527-320714 

Optimization of the biotransformation showed that the best conditions for achieving high rates and molar conversions included the use of n-hexane and -heptane as solvents, a temperature of 50 °C, an equimolar concentration of the substrates around 50 mM, and a concentration of the lyophilized biocatalyst around 25-30 g/1. As a matter of fact, Table 6.1 shows how the carbon source affects the expressed activity of A. oryzae MIM and R. oryzae CBS 112.07. 

Tween 80 proved to be the best carbon source for supporting the activity of lyophilized mycelia, while the use of more conventional carbon sources (glucose, sucrose, and glycerol) gave mostly extracellular Hpolytic activities. On the basis of these data, growth conditions could be optimized for mycelium-bound activity and, consequently, fermentations were scaled-up to SOL 

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