Sarcina lutea test

The Sarcina lutea test is the official US Food and Drug Administration (FDA) test for detecting penicillin residues in milk and dairy products (41). In this test, milk samples are placed in stainless steel cylinders on an agar plate seeded with Sarcina lutea ATCC 9341. As milk diffuses into the agar, inhibitors prevent the growth of the organism, causing a zone the width of which is a measure of the antibiotic concentration. The test is sensitive to about 0.006 g/ ml penicillin G, and confirmation of positive results can be performed by the addition of penicillinase. 

The assay of carbenicillin in biological fluids presents problems in that it may be accompanied by traces of benzylpenicillin (probably arising by a-decarboxylation of carbenicillin) to which the usual test organism, Sarcina lutea, is extremely... 

In the six-plate test, the sample is brought in a punch hole or a paper disc on each of the six test plates that have been inoculated with Bacillus cereus. Bacillus subtilis (pH 6), Bacillus subtilis (pH 8), Sarcina lutea, Escherichia coli, and Bacillus stearothermophilus, respectively (33). Once the samples have been introduced, the plates are incubated for the required time at the required temperature. If, on one of the plates, a zone of inhibition of 1.0 mm is observed around the disks or holes, the result of the test is considered positive, provided that interferences have been excluded. 

Another kidney prescreening procedure or emergency slaughtered animals is the Sarcina lutea kidney test (102), which has been in use in Netherlands since... 

In this test, an incision is made in sample kidneys into which paper discs are inserted and left for 30-60 min. The moistened discs are placed on agar plates seeded with Sarcina lutea ATCC 9341. The test is highly sensitive for penicillin but shows moderate sensitivity to sulfonamides, tetracyclines, macrolides, and aminoglycosides (103). It is insensitive to chloramphenicol (104). 

Initial studies on sulfonamides (114) were limited to qualitative assessment of the occurrence of sulfamerazine residues in muscle and interior organs of rainbow trout using Sarcina lutea (ATCC 9341) and Staphylococcus aureus (ATCC 6538P) as test organisms. The method involved application of fish fluids... 

The fermentation batches are incubated aerobically under stirring at 28°-30°C. At intervals the antibiotic activity is assayed microbiologically by the agar diffusion method using Sarcina lutea as the test organism. The maximum activity is reached after 96-120 h of fermentation. 

A microbiological assay was described for lincomycin in animal feed using an agar diffusion assay and an agar slant of Sarcina Lutea ATCC No. 9341 as the test organism for the analysis of lincomycin (42). 

Culture and Inoculum. Sarcina lutea (P. C. I. /lOOl) is grown for bulk inoculum and diluted inoculum in brain heart infusion (Section IV, 1). Prepare a 1-100 dilution from bulk inoculum just prior to the test. 

The effect of Caibopol, a polycaiboxylic binder, is due to its imi-exchange character. A separatimi of aminoglycoside samples of different origins with bioautographic detecticm was described (30). Usually 0.1-O.S p.g of the test substance was spotted test organisms were Bacillus subtilis, Sarcina lutea, and Mycobacterium phlei. 

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