Salmonella typhimurium tryptophan biosynthesis

The first two steps in the biosynthesis of tryptophan in Salmonella typhimurium involve the enzyme complex anthranilate synthase-phosphoribosyltransferase, which is a tetramer having two subunits of each enzyme. The anthranilate synthase catalyzes reaction (7) and the phos-phoribosyltransferase catalyzes two reactions the N-terminal portion cleaves glutamine to glutamate giving NH3 for the anthranilate synthase, while the C-terminal portion catalyzes reaction (8).3,1,312 All these reactions require M2+ cations. Orotate phosphoribosyltransferase binds four Mn2+ ions in a cooperative fashion kinetic data have been interpreted in a scheme where both metal-free and metal-containing enzyme catalyze the reaction.313... 

The bacterial reverse mutation test (Ames Test) investigates the ability of chemicals and drags to induce reverse (back) mutations in bacteria, which involves base pair substitutions additions and/or deletions (frameshift mutations) of one or a few DNA base pairs. The bacterial strains used in the test system have mutations in genes coding for enzymes required for the biosynthesis of the amino acids histidine (Salmonella typhimurium) and tryptophan (Escherichia coli). If... 

D-form [5962-30-1] Intermediate in the biosynthesis of tryptophan in cultures of Schizophyllum commune and mutants of E. coli, Aerobacter aerogenes and Salmonella typhimurium. 2,4-Dinitrophenylhydrazone Mp 214°. 5-Phosphate Anthranilic deoxyribulotide C12H16NO9P 349.233 Intermed. in Tryptophan biosynth. 5-Phosphate, Di-Na salt.Mp 177-181°. Hygroscopic. 

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