Salmonella typhimurium stability

Salmonella typhimurium (stabilized TCE, preincubation assay) Gene mutation McGregor et al. 1989... 

Sodium lactate (3-4%) in cooked beef is effective in limiting proliferation of Salmonella typhimurium, L. monocytogenes, and E. coli 0157 H7 (Miller and Acuff, 1994). Sodium lactate (3%) added to vacuum-packaged beef top rounds is effective as an oxidant and color stabilizer for cooked beef (Maca et al., 1999 Choi and Chin, 2003). 

Great attention has been paid to HAS and their safety application in plastics and coatings. The 4-unsubstituted 2,2,6,6-tetramethylpiperidine is considered as relatively toxic, the acute oral toxicity being about 1 g/kg. The substitution in position 4 (i.e. the general mode in the synthesis of HAS for polymer purposes) dramatically improves the situation. Therefore, commercial HAS like 28 (R = H), 34,35a or 35b were approved for stabilization of packaging materials in contact with food [307]. Some data are available on properties of TEMPO (2,2,6,6-tetramethylpiperidinyl-l-oxyl) and its 4-amino or 4-hydroxy derivatives. They were found to act as weak intrinsic direct mutagens in Salmonella typhimurium. TEMPO increases intracellular hydroperoxide concentration. This may indicate its pro-oxidative effect which does not result, however, in cellular toxicity [314]. 

Many authors have used the acronym ALS to refer to the enzyme that catalyzes the reaction of Fig. 2.1.1. However, since ALS refers specifically to the pathway leading to valine and leudne through the intermediate (S)-2-acetolactate, the designation AHAS better describes all products of the reaction. AHAS is present in bacteria, fungi, and plants. Many of the early kinetic, mechanistic, and structural studies were carried out with AHAS isolated and purified from enteric bacteria such as Escherichia coli and Salmonella typhimurium. Eukaryotic AHAS has proven more difficult to isolate and purify because of its reduced stability. Three AHAS isozymes have been characterized in bacteria - AHAS I, II, and III - whereas only one isozyme is known in fungi and plants. 

---