Saliva cortisol

Young, E.A., Aggen, S.H., Prescott, C.A., and Kendler, K.S. (2000a) Similarity in saliva cortisol measures in monozygotic twins and the influence of past major depression. Biol Psychiatry 48 70-74. 

Young EA, Breslau N. Saliva cortisol in posttraumatic stress disorder a community epidemiologic study. Biol. Psychiatry 2004 56 205-209. 

Observations About Baseline Cortisol Based on Single Estimates of Plasma or Saliva... 

Differential measurement of cortisol and cortisone in human saliva. (161)... 

Anfossi, L., C. Tozzi, C. Giovannoli, et al. 2002. Development of a non-competitive immunoassay for cortisol and its application to the analysis of saliva. Anal. Chim. Acta 468 315-321. 

A sensitive enzyme-immunoassay for plasma or saliva testosterone uses testosterone-lla-hemisuccinate antiserum, bound to cellulose to provide a solid phase for convenient separation. Testosterone-horseradish peroxidase conjugate was employed as the label.Another enzyme-immunoassay for testosterone employs a testosterone-3-(0-carboxymethyloxime)-pencillinase conjugate for competitive binding to the testosterone antiserum. 21-Amino-ll/3,17o -dihydroxypregn-4-ene-3,20-dione ( Cortisol 21-amine ) linked to alkaline phosphatase has been used ° in an effective enzyme-immunoassay for cortisol. 

In general most steroids of clinical interest can be measured in saliva. For some steroids, such as cortisol, estriol, and progesterone, the measurement of the sahvary concentration appears to be a reliable indicator of the free concentration in plasma. For others (e.g., testosterone, 17-hydroxyprogesterone, estradiol, and aldosterone) the chnical usefulness of salivary measurements has not yet been fully established. 

Measurement of Free Cortisol in Blood, Urine, and Saliva... 

Salivary Specimens. Cortisol is stable in saliva for 1 week at 4 °C and for 4 months when stored frozen. Freezing of specimens is recommended because it leads to precipitation of salivary glycoproteins and leaves a nonviscous liquid for pipetting. ... 

Morineau G, Radioimmunoassay of cortisone in serum, urine, and saliva to assess the status of the cortisol-cortisone shuttle. Clin Chem 1997 43 1397-407. 

Wade, S.E. Haegele, A.D. Differential measurement of cortisol and cortisone in human saliva by HPLC with UV detection. J.Liq.Chromatogr., 1991, 14, 1813-1827 [saliva UV detection LOD 0.5 ng/mL column-switching column temp 50 SPE]... 

The measurement of stress-related hormonal changes has been used in various ergonomic studies (see chap 16, this volume). Catecholamines (epinephrine and norepinephrine) and cortisol are the most fiequentiy used stress hormones. Because blood samples are difScult to obtain during work, analyses of urine fractions are preferred. However, these cannot indicate elTeets of short-term strain. Epinephrine indieates long-lasting mental, and sometimes emotional, strain, whereas norepinephrine acts as an indicator of physical strain. Cortisol can now be recorded, with sufficient reliability, from saliva probes. 

Jonsson, B.A. Malmberg, B. Amilon, A. Helene Garde, A. Orbaek, P. Determination of cortisol in human saliva using liquid chromatography-electrospray tandem mass spectrometry. J. Chromatogr. B, 2003, 784 (1), 63-68. 

Can be easily obtained from infant saliva, but since cortisol production follows a circadian rhythm and level of salivary cortisol can be influenced by recent intake of milk or milk products, assessment requires controls for time of day and milk exposure (Posner, 2001)... 

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