Saliva testosterone

A sensitive enzyme-immunoassay for plasma or saliva testosterone uses testosterone-lla-hemisuccinate antiserum, bound to cellulose to provide a solid phase for convenient separation. Testosterone-horseradish peroxidase conjugate was employed as the label.Another enzyme-immunoassay for testosterone employs a testosterone-3-(0-carboxymethyloxime)-pencillinase conjugate for competitive binding to the testosterone antiserum. 21-Amino-ll/3,17o -dihydroxypregn-4-ene-3,20-dione ( Cortisol 21-amine ) linked to alkaline phosphatase has been used ° in an effective enzyme-immunoassay for cortisol. 

In general most steroids of clinical interest can be measured in saliva. For some steroids, such as cortisol, estriol, and progesterone, the measurement of the sahvary concentration appears to be a reliable indicator of the free concentration in plasma. For others (e.g., testosterone, 17-hydroxyprogesterone, estradiol, and aldosterone) the chnical usefulness of salivary measurements has not yet been fully established. 

Salivary testosterone concentration correlates with free testosterone concentrations in plasma and has been advocated as an indicator of androgen status. In a multicenter evaluation that examined nine different laboratories using various RIA methods with and without extraction, good correlation was found between laboratories despite widely varying methods. Reference intervals of 4 to 14ng/dL and 2 to 6ng/dL have been reported for testosterone in saliva of men. Measurement of salivary testosterone in women has been less reliable, with no consensus on salivary concentrations available. However, one commer-... 

Shirtcliff EA, Granger DA, Likos A. Gender differences m the validity of testosterone measured in saliva by immunoassay. Horm Behav 2002 42 62-9. 

A Turkes, AO Turkes, BG Joyce, GF Read, D Riad-Fahmy. A sensitive solid phase enzymeimmunoassay for testosterone in plasma and saliva. Steroids 33 347, 1979. 

An enzyme-immunoassay for testosterone in female plasma and saliva uses lla-hydroxytestosterone 11-hydrogen succinate-horseradish peroxidase conjugate as enzyme label, and p-hydroxyphenylacetic acid to provide a fluorimetric end-point . A solid-phase enzyme-immunoassay has been described for 19-norethisterone, based upon the lla-hydrogen succinoyloxy derivative. ... 

Analysis of Testosterone and De-hydroepiandrosterone in Saliva by Gas Chromatography-Mass Spectrometry... 

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