SAGE libraries

More than 300 transcripts, identified in treatment comparisons, had a five-fold or greater difference in abundance. The ten most abundant SAGE tags identified in the UV-treated SAGE library are listed in Table 11.1. Greater than... 

Table 11.1 The ten most abundant SAGE tags identified in the UV-treated SAGE library.

Follow the tag hotlinks in the output table to find out its expression level in different SAGE libraries and how it is represented in the rest of the sequences in GenBank and UnlGene. 

Figure 16.6. The complete list of tags from the virtual Northern results (Figs. 16.4 and 16.5) can also be used to query the database, finding the occurrence of their tags in all SAGE libraries.

Figure 16.7. (a) The xProfiler tool allows the user to perform an electronic subtraction between sets of SAGE libraries by selecting libraries in this window. In this example, normal colonic epithelium Is subtracted from colon cancer, (b) The results of the electronic subtraction are displayed in tabular form. 

Cirlot, Juan Eduardo. A dictionary of symbols. Translated from the Spanish by Jack Sage. Foreword by Herbert Read. 2nd ed ed. Edited by Herbert Read. Translated by Jack Sage. New York Philosophical Library, 1971. lv, 419 p. 

Building up expertise in this field, EMBL with its data library is well positioned to play an important European role in estabfishing a reference DNA array and SAGE database. In the future, the micro-array technology will be developed also for proteins, applying miniaturization, nanotechnology, micro-fabricated devices and reaction chambers. 

Atul Kohli read Chapters I and V of an earlier draft. Avner Ben-Ze ev, David Cohen, Joseph Frank, David Lai tin, Robert Merton, and Roger Petersen read the entire draft. I benefited greatly from their comments. I am particularly indebted to Robert Merton for urging me to write the Coda. Finally, I want to thank Aida Llalaby for her Invariably friendly and competent assistance, my research assistant Joshua Rosenstein, as well as Cheryl Seleski and the marvelously efficient library staff at the Russell Sage Foundation, which provided me with a fellowship to finish this book. 

While cDNA libraries can be used to obtain many genes in a short time frame, it may not yield genes of toxicologic importance. To enrich for these genes there are several methods that rely on obtaining mRNAs that are differentially expressed after treatment. These methods, which are described below, include subtractive hybridizations, DD RT-PCR and SAGE. 

Figure 16.1. Serial Analysis of Gene Expression (SAGE) depends on the generation of a tag from the 3 end of an mRNA, Tags are concatemerized and sequenced. These data are compared with a database of tags linked to individual transcriptsto generate the frequency of each tag in the library, a measure of the expression level for that gene. (See color plate.)...

University of California San Diego Science and Engineering Library Materials Science Guide http //libraries.ucsd.edu/sage/subject subject=10 (accessed September 29,2010). 

---