Saccharomyces cerevisiae batch cultivations

Keywords Ethanol Cashew apple Juice Saccharomyces cerevisiae Batch cultivation Kinetic parameters... 

Hansenula polymorpha and Saccharomyces cerevisiae were cultivated on synthetic medium with 1% glucose in fed-batch and continuous mode, respectively, in the absence of antifoam agents. For the nutrient preparation, sterilization and storage, 300-, 600-, 1000- and 5000-1 stirred tank vessels were used. The nutrient salt medium was steriUzed without glucose. The glucose solution was autoclaved separately and was added to the cold, sterilized nutrient medium. The flotation column was operated in continuous mode. 

These optical probes are the most universally applicable in situ devices for on-line biomass monitoring up to now [15,16]. Konstaninov et al. [17] tested several absorbance and scattering sensors for real-time biomass concentration monitoring in mammalian cell cultivation processes and Hatch and Veilleux [18] compared optical density probes with oxygen uptake rates, packed cell volume, and off-line cell mass monitoring in commercial fed-batch fermentations of Saccharomyces cerevisiae [19]. In order to minimize influencing effects, special chemometric data treatment is necessary [20]. 

Arndt M, Hitzmann B (2004). Kalman filter based glucose control at small set points during fed-batch cultivation of Saccharomyces cerevisiae. Biotechnol. Prog. 1 377-383. 

Jin et al. applied fuzzy control and artificial neural networks for fed-batch cultivation of recombinant saccharomyces cerevisiae to improve productivity and product yield of P-galactosidase. Experimental data have shown that by application of fuzzy control and neural network estimators, the productivity was 2.7-fold higher than in the case of exponential feeding. 

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