Sabouraud broth

Every 4 months, open a new ampoule and subculture to Sabouraud dextrose broth (SDB). Unless otherwise stated, the incubation conditions for this organism are 24 to 48 hours at 30°C. Subculture from the SDB to Sabouraud dextrose agar (SDA) slopes (stock slopes) and concurrently plate onto an SDA plate to check for purity. Prepare sufficient stock slopes to last 4 months. 

Standard nutrient media for bacterial (Luria Broth Miller, Muller-Hinton Broth) and fungal growth (Sabouraud, yeast culture media). 

The next step is to gently rub the ulcer with a sterile calcium alginate swab moistened with thioglycolate broth or trypticase soy broth and inoculate three rows of C on each of the blood, chocolate, and Sabouraud s plates. This swab is placed in a tube of thioglycolate broth medium to isolate anaerobes. A freshly moistened swab should then be rubbed across the corneal ulcer and used... 

The TAC can be conducted using a number of microbiological methods. These are the pour-plate, membrane filtration, and multiple tube methods. The TYMC is conducted by using either the pour-plate or membrane filtration method. The TAC for bioburden is performed by adding 10 g, 10 mL, or 10 units in SCD broth or lactose broth to make 100 mL. Aliquots of this sample preparation are transferred into four standard size (15 x 100 mm) Petri dishes. Into two of the plates 15-20 mL of molten SCD agar is poured, and into the other two the same volume of Sabouraud dextrose agar (SAB) agar is poured. 

The broth dilution method was used in these studies. Pseudomonas organisms were cultivated in I per cent peptone, 3 per cent NaCi medium, and Dipl, pneumoia. Sir, pyo nes and CoU. Dipktk riae were cultivated on heart infusion broth containing 0 5 per cent glucose. All other bacteria were cultivated on heart infusion broth. F gi were grown in Sabouraud s medium. 

In order to determine the optimum pore diameter range for mycelial growth, 0.5 grams of each carrier with the immobilized spores was placed in 75 ml of Sabouraud dextrose broth and the composite was allowed to shake on a shaker at room temperature. At the end of 27 hours, a sample of each carrier was taken and the amount of ATP was determined as a measure of mycelial growth. The results are recorded in Figure 6. 

Both culture media must be sterilised by heating 20 min at 120° C in an autoclave. The Sabouraud medium is suitable especially for culture of fungi. In the preparation of the agar culture medium, 10 g meat extract and 1000 ml distilled water can be used instead of broth. 

Inquiries into the possible production of behavioral chemicals of D. frontalis by microorganisms associated with it have been carried further. As mentioned above, two fungi and two yeasts are associated with the mycangium of the female (440, 441). The production of various volatile substances, other than ethanol, by actively fermenting yeasts is well established (445). Brand et al. (446) grew three yeasts obtained from D. frontalis, namely H. holstii, P. pinus, and P. bovis, on Sabouraud s dextrose broth, and identified isoamyl alcohol, 2-phenylethanol, isoamyl acetate and 2-phenylethyl acetate as the main volatile substances (other... 

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