Runs, methamphetamine

A somewhat different view is supported by a number of other studies. Behavioural rhythmicity persists in SCN-lesioned rodents under the influence of either periodically available food (Davidson Stephan 1999, Stephan et al 1979) or continuous treatment with methamphetamine (Honma et al 1992). Rhythmicity of at least some circadian genes persists in isolated peripheral tissues for periods of time ranging from several days to two or three weeks (Yamazaki et al 2000, our unpublished results). Removal of the retina (which is known to contain its own circadian oscillator Tosini Menaker 1996) changes the distribution of free-running periods of locomotor activity and the duration of active time (a) (Yamazaki et al 2002a). To pursue our metaphor, these results suggest that circadian signals may be relayed to the periphery by a baton rather than a whip the SCN may indeed be the conductor of an orchestra composed of dozens, if not thousands of potentially independent oscillators. 

Figure 9.3 Effect of SDS added to the run buffer on the separation of (1) ephedrine, (2) pseudoephedrine, and (3) methamphetamine on S-folded separation channel (145 mm length) using 50 mM phosphate buffer (pH 7.35) with 10 mM HS-y-cyclodextrin at 8 kV/cm potential, (a) 10 mM HS-y-CD and (b) 10 mM HS-y-CD [18].

Strong bases (amphetamines, methamphetamine, procaine), bases of medium strength (cocaine, heroin, noscapine, quinine), weak bases (diazepam, methaqualone), strong acids (A-9-tetrahydrocannabinolic acid), moderately strong acids (phenobarbi-tal) and weak acids (A-9-tetrahydrocannabinol) can be separated in a single run along with neutral solutes (testosterone, testosterone propionate). However, in order to... 

Hifumi et al. [34] applied ultra-fine platinum particles protected by poly(Me acrylate-co-N-vinyl-2-pyrrolidone) to inununological detection of metham-phetamine (MA). The polymer-protected ultra-fine particles chemically boimd anti-methamphetamine monoclonal antibody to their surfaces. The antibody-fixed particles behaved like an antibody in the inununoreaction, making it possible to detect the MA to a concentration of ca. 10 ng/mL. Uda et al described a similar application of polymer-protected ultra-fine platinum particles to the im-mvmological detection of human serum albumin [35]. Tamai et al. [36] showed that ultra-fine metal particles could be immobilized on fine copolymer particles that were produced by reducing copolymer particles-metal ion complexes. Transmission electron microscopy and X-ray diffraction were used to confirm that ultra-fine noble metal particles with a diameter below 10 run were formed and uniformly immobilized on the surface of copolymer particles. 

UV electropherograms of ( ) methamphetamine, ( )-methcathinone, ( )-ephedrine and ( )-pseudoephedrme standards. The sample concentrations were 25 ppm. CE conditions capiilary, 58.5 cm (50 cm to detector) detection wavelength, i t = 210 nm. Running buffers (a) 150 mM H PO wateracetonitrile = 95 5 (v/v) (b) the same buffer as described earlier and the addition of 17.5 mM b-CD. The inset shows the separation of ( )-methamphetamine, ( )-methcathinone, ( )-ephedrine, ( )-pseudoephedrine and ( )-ampheta-mine standards when and 80 cm capillary was used. Reprinted from reference 3 cited below, copyright 2003, with permission from Elsevier. 

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