Run, Diffusion of Antiserum, and Drying

In order to gain visual control during the electrophoretic migration, we have found the following technique satisfactory (W8). One indicator consists of a 35 % sol of dextran (mol. wt. 150,(XK)) colored a deep blue with bromophenol blue. A small drop is placed on the agar near the serum sample. Part of it will dye the serum albumin exclusively, since the serum globulins are only colored after denaturation. Thus, the albumin front will take on a pure bluish color and demonstrate anionic progress. 

The other indicator has the same dextran as colloid base, but is colored 

Now the plastic cell is closed to reduce evaporation. The electric current is switched on again for the nm. An individual voltmeter and milliammeter are useful. 

When separation has proceeded long enough, the current is stopped and the agar is lifted out of the longitudinal cuts. Into these narrow channels antiserum is added from a micropipet connected to rubber tubing. Care must be taken that it is distributed evenly along the whole 

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