Ribosomal spacer genes

Kane, R.A. and Rollinson, D. (1998) Comparison of the intergenic spacers and 3 end regions of the large subunit (28S) ribosomal RNA gene from three species of Schistosoma. Parasitology 1 1 7, 235-242. 

Cunningham, C.O. (1997) Species variation within the internal transcribed spacer (ITS) region of Gyrodactylus (Monogenea Gyrodactylidae) ribosomal RNA genes. Journal of Parasitology 83, 21 5-21 9. 

KOTOB s, MCLAUGHLIN SM, VAN BERKUM p and FAISAL M (1999), Discrimination between two Perkinsus spp isolated from the softshell clam, Mya arenaria, by sequence analysis of two internal transcribed spacer regions and the 58S ribosomal RNA gene, Parasitology, 119, 363-368. 

The genes for 5S ribosomal RNA and all of the tRNAs are transcribed by RNA polymerase III. In the yeast genome die 5S RNA genes are located in the spacers between the transcriptional units containing the other rRNAs. However, in animals the 5S RNA... 

Ribosomes, the intracellular particles on which proteins are assembled, are highly complex and dynamic entities. The structural framework of ribosomes is provided by ribosomal RNA (rRNA) molecules with which many proteins are associated (summarized in Capowski and Tracy, 2003). Homologous rRNA genes occur in all prokaryotes and eukaryotes. The mitochondrial and chloroplast rRNA genes in eukaryotes clearly have prokaryote affinities (Pace et ai, 1986). The genomic DNA from which ribosomal genes are transcribed, along with any associated spacers, is collectively termed ribosomal DNA (rDNA). Sequences and other data from rDNA and its products,... 

E. coli contains clusters of up to seven tRNA genes separated by spacer regions, as well as tRNA genes within ribosomal RNA transcription units. Following transcription, the primary RNA transcript folds up into specific stem-loop structures and is then processed by ribonucleases D, E, F and P in an ordered series of reactions to release the individual tRNA molecules. 

Fernandez-Espinar, M.T., Esteve-Zarzoso, B., Querol, A., Barrio, E. (2000). RFLP analysis of the ribosomal internal transcribed spacers and the 5.8S rRNA gene region of the genus Saccharomyces-. a fast method for species identification and the differentiation of flor yeasts. Antonie van Leeuwenhoek, 78, 87-97. 

Garcia-Martinez,., Acinas, S. G., Anton, A. I., and Rodriguez-Valera, F. (1999). Use of the 16S-23S ribosomal genes spacer region in studies of prokaryotic diversity.J. Microbiol. Methods 36, 55-64. 

The discovery that rRNA plays an active role in all the functions of the ribosome, as well as the observation that there is a core in the rRNA molecules that has been conserved in all organisms, has given strong support to Woese s proposal [75] that the most primitive ribosomes were composed solely of RNA. On the genetic level, the identification of internal transcribed spacers (ITS) [47] or intervening sequences (IV S) [48] in the rRNA genes of bacteria and eucarya has provided us with a... 

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