Internal transcribed spacer regions

Takashima M, Nakase T Molecular phylogeny of the genus Cryptococcus and related species based on the sequences of 18S rDNA and internal transcribed spacer regions. Microbiol Cult Coll 1999 15 35 7. 

Sugita T, Takashima M, Ideka R, Nakase T, Shinoda T Intraspecies diversity of Cryptococcus laurentii as revealed by sequences of internal transcribed spacer regions and 28S rDNA gene and taxonomic positions of C. laurentii clinical isolates. J Clin Microbiol 2000 38 1468-1471. 

Trcek, J. 2005. Quick identification of acetic acid bacteria based on nucleotide sequences of the 16S-23S rDNA internal transcribed spacer region and of the PQQ-dependent alcohol dehydrogenase gene. Systematic Applied Microbiology 28 735-745. 

Renker, C., Heinrichs, J., Kaldorf, M., Buscot, R, 2003. Combining nested PCR and restriction digest of the internal transcribed spacer region to characterize arbuscular mycorrhizal fungi on roots from the field. Mycorrhiza 13, 191-198. 

Ji Y-J, Zhang D-X, He L-J (2003). Evolutionary conservation and versatility of a new set of primers for amplifying the rihosomal internal transcribed spacer regions in insects and other invertebrates. Mol Ecol Notes, 3 581-585. 

DNA-based approach (phylogenetic approach) has been reported by Canadian investigators for various species of psilocybin mushrooms which are quite helpful in the identification of species that contain the psychoactive compounds [153]. The method includes DNA amplification and sequencing of the internal transcribed spacer region of the rDNA (ITS-1) and a 5 portion of the nuclear large ribosomal... 

Egli, G.M. and T. Henigk-Kling. 2001. Identification of Brettanomyces/Dekkera species based on polymorphism in the rRNA internal transcribed spacer region. Am.J. Enol. Vitic. 52 241—247. 

Figure 2. PCR amplification of the internal transcribed spacer regions of five Chironomid species. Using primers specific to the 18S and 28S regions of rDNA, five different Chironomid species yield amplification products of characteristic lengths. Lane A - Glyptotendipes lobiferous, Lane B -Cardiocladiiis obscurus, Lane C - Chironomus riparius, Lane D - Dicrotendipes fumidus, Lane E - Cricotopus bicinctus, and Lane SM -100 bp size marker.

Liston, A., W.A. Robinson, D. Pinero and E.R. Alvarez-BuyUa. 1999. Phylogenetics of Pinus (Pinaceae) based on nuclear ribosomal DNA internal transcribed spacer region sequences. Mol. Phylogenet. Evol. 11 95-109. 

Gernandt, D.S. and A. Liston. 1999. Internal transcribed spacer region evolution in Larix and Pseudotsuga. Am. J. Bot. 86 711-723. 

KOTOB s, MCLAUGHLIN SM, VAN BERKUM p and FAISAL M (1999), Discrimination between two Perkinsus spp isolated from the softshell clam, Mya arenaria, by sequence analysis of two internal transcribed spacer regions and the 58S ribosomal RNA gene, Parasitology, 119, 363-368. 

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