Ribonuclease enzymatic hydrolysis

Protoplasts, produced by enzymatic hydrolysis of the cell walls, have been used as a source of plant organelles and could be used as a source of soluble enzymes, but the methods can only be applied to relatively soft tissues and require prolonged exposure of tissues to cell wall degrading enzymes >. In addition, most of the fungal enzyme preparations that ea e used contain such undesirable products as proteases, ribonucleases, peroxidases and phenolics. ... 

ESI-MS has been used for the quantification of a number of substrates and products of enzymatic reactions [56,57]. Hsieh et al. report the use of ion spray mass spectrometry (a technical variation of electrospray ionization) coupled to HPLC for the kinetic analysis of enzymatic reactions in real time [58]. The hydrolysis of dinucleotides with bovine pancreatic ribonuclease A and the hydrolysis of lactose with 3-galactosidase were monitored and the resulting data were used for the estimation of and v x of these reactions. Another field of application of electrospray mass spectrometry is the screening of combinatorial libraries for potent inhibitors [31,59]. 

The tertiary structure of a protein refers to the arrangement of amino acid side chains in the protein. Generally, the information for protein structure is contained within the amino acidjequence of the protein itself This important principle of biochemistry was first determined by the biochemist Christian Anfinsen in studies of the enzyme ribonu-clease. Ribonuclease catalyzes a simple hydrolysis of ribonucleic acid. The native enzyme has 124 amino acids 8 of these are cysteines, forming 4 disulfide bonds. When ribonuclease was treated with mercaptoethanol to destroy the disulfide bonds and urea to disrupt its secondary and tertiary structure, all enzymatic activity was... 

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