RFLPs bacterial

Mycobacteria of the Mycobacterium avium complex are implicated in disseminated bacterial infections in AIDS patients. RFLP studies followed by hybridization with radiolabeled probe specific for an insertion sequence in M. avium (IS 1311) have been useful for typing M. avium stains (R2). A variety of molecular techniques are available for the diagnosis of Chlamydia trachomatis infection. In addition to PCR, a method based on the ligase chain reaction has also been found to be sensitive to the detection of C. trachomatis infection in urine specimens collected from male and female subjects (VI). The differentiation between low-risk genotypes of human papilloma virus (HPV 6 or 11) from genotypes of high... 

Covert and Moran (2002) Marine Whole, HMW, and LMW DOM Bacterial respiration 16S rDNA clone libraries and T-RFLP No 6 weeks LMW DOM 4-1 Ox more bioavailable HMW DOM community dominated by a- and p-Proteobacteria LMW DOM community dominated by y- and 8-Proteobacteria... 

T-RFLP has been used to study microbial diversity in contaminated sediments and to monitor changes in community composition following exposure to pollutants. In a study by Covert and Moran [77], the structure of bacterial communities growing at high-molecular weight (HMW) and at low-molecular weight (LMW) dissolved organic carbon was compared by T-RFLP. 

The results showed a dominance of gamma and epsilon Proteobacteria in the case of LMW but a dominance of alpha, beta, and gamma Proteobacteria in addition to members of the Cytophaga/Flavobacterium/Bacteriodes group in the case of HMW. Konstantinidis et al. [78] used T-RFLP in order to estimate the bacterial diversity in sediments contaminated with heavy metals. Their analysis revealed a conserved community structure over a depth profile of 15 cm. 

T-RFLP could also be used to follow shifts in bacterial community structure. Turpeinen et al. [79], used this technique to study the impact of contamination by arsenic, chromium and copper on microbial communities. The T-RFLP analysis showed that contamination with metals induces permanent changes in the microbial community structure. Rousseaux et al. [80] also re-... 

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